Cell-free synthesis of amyloid fibrils with favorable functional and analytical features

Jul 24, 2023 | Cell-Free, Proteins

Les amyloïdes sont des agrégats de protéines présentant une structure fibrillaire non ramifiée typique, qui sont impliqués dans plusieurs maladies de mauvais repliement des protéines, associées à des maladies neurodégénératives telles que les maladies...

Cell-free expressed OprF proteoliposomes as effective vaccine against Pseudomonas aeruginosa?

Mar 25, 2022 | Cell-Free, Proteins, Proteoliposomes

Synthelis’ cell-free liposome-based expression system was applied in the development of an OprF vaccine against Pseudomonas aeruginosa. The complete OprF outer membrane protein of P. aeruginosa was used as the immunogen and was expressed in its native form in...

Proteoliposomes – ideal model systems for membrane protein analysis

Mar 25, 2022 | Proteins, Proteoliposomes

Proteoliposomes – ideal model systems to study the structure and function of membrane proteins in semi-native environment by cryogenic electron microscopy What are Proteoliposomes ? Proteoliposomes are lipid vesicles containing membrane proteins that can be used as...

The S-protein receptor binding domain (RBD) potential uses for SARSCoV immunization and detection

Mar 20, 2022 | Proteins, RBD protein, SARS-CoV-2

The S-protein receptor binding domain (RBD) of SARS-CoV-2 has been the subject of a thorough characterization and its importance in virus infection is well known. The potential uses of RBD of S-protein as a vaccine and as an antigen for the specific and sensitive...

Five reasons to label your proteins for NMR studies using cell-free systems

Mar 20, 2022 | Cell-Free, Proteins

Isotope labelling has been used for over 40 years to facilitate the study of protein structure using nuclear magnetic resonance (NMR) spectroscopy. During that time, traditional expression systems such as in vivo protein synthesis were the preferred options for...