Cell-free production of adeno-associated virus protein particles

May 15, 2025 | Blog, Cell-Free, Proteins, Uncategorized

Cell-free production of adeno-associated virus protein particles – Expanded horizons for gene therapy and vaccine development Virus-like proteins (VLPs) are non-infectious polymeric nanoparticles formed from virus-coat proteins but deprived of genetic material. These...

Des systèmes acellulaires pour produire des particules pseudovirales !

Oct 3, 2023 | Blog, Cell-Free

Les particules pseudovirales (VLP) sont des nanostructures constituées de protéines virales assemblées, dépourvues de matériel génétique viral et donc non infectieuses. Leur intérêt réside dans la possibilité de les utiliser pour transporter et délivrer des...

Cell-free synthesis of amyloid fibrils with favorable functional and analytical features

Jul 24, 2023 | Cell-Free, Proteins

Les amyloïdes sont des agrégats de protéines présentant une structure fibrillaire non ramifiée typique, qui sont impliqués dans plusieurs maladies de mauvais repliement des protéines, associées à des maladies neurodégénératives telles que les maladies...

New trends in cell-free Synthetic Biology

May 15, 2023 | Cell-Free

Cell-free Synthetic Biology takes use of biomolecular reactions outside cells to synthesise specific products with applications in medicine, agriculture, environment and industry, particularly in the fields of drug discovery, diagnostics, biomanufacturing and...

Cell-free systems are only relevant for protein synthesis

Mar 26, 2022 | Cell-Free Biomyths

Cell-free systems are mainly used for in-vitro protein synthesis and can be considered as a powerful tool in genetic code reprogramming, involving the amber codon. In this use, the non- proteinogenic amino-acids are incorporated into proteins by charging them to...

Cell-free systems are complex; hence the increased uptake in recent years of cell-free kits

Mar 26, 2022 | Cell-Free Biomyths

The wide availability and convenience of cell-free kits has led to their increased use over the last few years. However, despite the multitude of cell-free kits on the market, they do not allow as much flexibility as custom solutions. Indeed, the base reaction buffer...

Cell-free systems do not produce functional protein complexes

Mar 26, 2022 | Cell-Free Biomyths

Cell-free systems not only enable the production of proteins, but also protein complexes composed of around 2 to 1500 proteins. Indeed, the open nature of cell-free systems is advantageous for the co-expression of proteins since the ratios of DNA templates and the...

Cell-free systems only work with plasmids

Mar 26, 2022 | Cell-Free Biomyths

It is true that cell-free protein synthesis works with genetic materials such as plasmid DNA but also with PCR-amplified linear DNA sequences. However, when cell-free expression assays started with PCR fragments, the expression yields were much lower than from plasmid...

Cell-free systems are only relevant for membrane proteins

Mar 26, 2022 | Cell-Free Biomyths

Cell-free protein synthesis (CFPS) methodology offers the possibility to produce proteins that would be difficult or even impossible to synthesize in cell-based systems. These so-called “difficult-to-express proteins” include membrane proteins, but also cytotoxic...

Cell-free systems deliver low expression yields

Mar 25, 2022 | Cell-Free Biomyths

It is generally considered that cell-free protein synthesis systems are capable of generating only a small expression yield. Even if this was true in the 2000s, it is certainly no longer the case today (Carlson et al., 2012) as we will explain in the following paper....