Syn-Xpress™ Gold VHH
Cell-Free Kit

The Syn-Xpress™ Gold VHH kit is designed for users seeking a simple, fast, and reliable solution to produce their VHH or mini-binder using cell-free protein synthesis (CFPS).
It contains the cell lysate and a complete energy mix (nucleotides, energy source, amino acids, salts, and cofactors), pre-formulated to ensure optimal performance and enable the formation of disulfide bonds in your VHH or mini-binder.

With Syn-Xpress™ Gold VHH, you only need to add your expression DNA (plasmid or linear). This configuration provides a significant time saving while ensuring high reproducibility.

Each Syn-Xpress™ Gold VHH kit comes with a control plasmid encoding Syn-GFP, an optimized GFP for cell-free expression. This control allows you to validate your experimental conditions.

The Syn-Xpress™ Gold VHH kit includes:
. The mastermix, containing the lysate and the complete energy mix
. A control plasmid tube encoding Syn-GFP
. A user manual

Notes:

The 96 × 50 µL format is supplied directly in a 96-well plate; other formats are supplied in tubes.
The kits are composed of 90% mastermix, leaving 10% of the final reaction volume for adding your DNA and nuclease-free water to reach the desired total volume.
For example, 50 µL reactions contain 45 µL of mastermix, and 1 mL reactions contain 900 µL of mastermix.

Other formats are available upon request, enabling high-throughput screening or scale-up. When scaling up, the system’s yields are linear.

With Syn-Xpress™ Gold VHH, produce your own VHHs:

Easily: simply add your DNA.
Quickly: obtain results within a few hours, eliminating cloning and bacterial transformation steps.
Specifically: precisely validated for VHH domains and mini-binders.
With easy purification: compatible with affinity tags (His-tag, Strep-tag, etc.) and free from other tagged proteins, ensuring specific and rapid purification of your target protein.
At a lower cost than with an in vivo system.