Cell-free systems to produce Virus-Like Particles!

Oct 3, 2023 | Blog, Cell-Free

Les particules pseudovirales (VLP) sont des nanostructures constituées de protéines virales assemblées, dépourvues de matériel génétique viral et donc non infectieuses. Leur intérêt réside dans la possibilité de les utiliser pour transporter et délivrer des...

Cell-free synthesis of amyloid fibrils with favorable functional and analytical features

Jul 24, 2023 | Cell-Free, Proteins

Les amyloïdes sont des agrégats de protéines présentant une structure fibrillaire non ramifiée typique, qui sont impliqués dans plusieurs maladies de mauvais repliement des protéines, associées à des maladies neurodégénératives telles que les maladies...

New trends in cell-free Synthetic Biology

May 15, 2023 | Cell-Free

Cell-free Synthetic Biology takes use of biomolecular reactions outside cells to synthesise specific products with applications in medicine, agriculture, environment and industry, particularly in the fields of drug discovery, diagnostics, biomanufacturing and...

Cell-free protein synthesis systems the best chance for your project to succeed!

Mar 25, 2022 | Cell-Free

Do you have a protein you want to express? Are you wondering which technology will provide you with the best results? Have you considered turning to a cell-free system? In comparison to traditional protein expression systems, cell-free protein synthesis can provide a...

Cell-free expressed OprF proteoliposomes as effective vaccine against Pseudomonas aeruginosa?

Mar 25, 2022 | Cell-Free, Proteins, Proteoliposomes

Synthelis’ cell-free liposome-based expression system was applied in the development of an OprF vaccine against Pseudomonas aeruginosa. The complete OprF outer membrane protein of P. aeruginosa was used as the immunogen and was expressed in its native form in...

Proteoliposomes – ideal model systems for membrane protein analysis

Mar 25, 2022 | Proteins, Proteoliposomes

Proteoliposomes – ideal model systems to study the structure and function of membrane proteins in semi-native environment by cryogenic electron microscopy What are Proteoliposomes ? Proteoliposomes are lipid vesicles containing membrane proteins that can be used as...

The nucleocapsid protein of SARS-CoV-2potential target for a vaccine

Mar 25, 2022 | SARS-CoV-2

What is the N-Protein ?The nucleocapsid protein (N protein) of SARS-CoV-2 is a structural protein synthesized by the virus, which plays a key role in the RNA packaging into the nucleocapsid. Its function is to mediate viral assembly through interaction with the viral...

The S-protein receptor binding domain (RBD) potential uses for SARSCoV immunization and detection

Mar 20, 2022 | Proteins, RBD protein, SARS-CoV-2

The S-protein receptor binding domain (RBD) of SARS-CoV-2 has been the subject of a thorough characterization and its importance in virus infection is well known. The potential uses of RBD of S-protein as a vaccine and as an antigen for the specific and sensitive...

Which extract is best for cell-free protein expression wheat germ or E. coli?

Mar 20, 2022 | Biotech, Cell-Free

Cell-free protein expression offers a rapid, reliable technique for synthesis of proteins. Successfully synthesizing large quantities of high-quality product requires finding the optimal protein expression conditions, such as the type of cellular extract used. Lysate...

Five reasons to label your proteins for NMR studies using cell-free systems

Mar 20, 2022 | Cell-Free, Proteins

Isotope labelling has been used for over 40 years to facilitate the study of protein structure using nuclear magnetic resonance (NMR) spectroscopy. During that time, traditional expression systems such as in vivo protein synthesis were the preferred options for...