Current challenges and perspectives in proteoliposome research
Membrane proteins (MPs) are very important in cells as they accomplish a diversity of roles, justifying the increasing interest in their study. However, functional studies of MPs within a native cellular setting are difficult to perform due to the innate complexity of...
Cell-free synthesis of amyloid fibrils with favorable functional and analytical features
Les amyloïdes sont des agrégats de protéines présentant une structure fibrillaire non ramifiée typique, qui sont impliqués dans plusieurs maladies de mauvais repliement des protéines, associées à des maladies neurodégénératives telles que les maladies...
Cell-free expressed OprF proteoliposomes as effective vaccine against Pseudomonas aeruginosa?
Synthelis’ cell-free liposome-based expression system was applied in the development of an OprF vaccine against Pseudomonas aeruginosa. The complete OprF outer membrane protein of P. aeruginosa was used as the immunogen and was expressed in its native form in...
Proteoliposomes – ideal model systems for membrane protein analysis
Proteoliposomes – ideal model systems to study the structure and function of membrane proteins in semi-native environment by cryogenic electron microscopy What are Proteoliposomes ? Proteoliposomes are lipid vesicles containing membrane proteins that can be used as...
The S-protein receptor binding domain (RBD) potential uses for SARSCoV immunization and detection
The S-protein receptor binding domain (RBD) of SARS-CoV-2 has been the subject of a thorough characterization and its importance in virus infection is well known. The potential uses of RBD of S-protein as a vaccine and as an antigen for the specific and sensitive...