Cell-free production of adeno-associated virus protein particles
Cell-free production of adeno-associated virus protein particles – Expanded horizons for gene therapy and vaccine development Virus-like proteins (VLPs) are non-infectious polymeric nanoparticles formed from virus-coat proteins but deprived of genetic material. These...
Des systèmes acellulaires pour produire des particules pseudovirales !
Les particules pseudovirales (VLP) sont des nanostructures constituées de protéines virales assemblées, dépourvues de matériel génétique viral et donc non infectieuses. Leur intérêt réside dans la possibilité de les utiliser pour transporter et délivrer des...
Cell-free synthesis of amyloid fibrils with favorable functional and analytical features
Les amyloïdes sont des agrégats de protéines présentant une structure fibrillaire non ramifiée typique, qui sont impliqués dans plusieurs maladies de mauvais repliement des protéines, associées à des maladies neurodégénératives telles que les maladies...
New trends in cell-free Synthetic Biology
Cell-free Synthetic Biology takes use of biomolecular reactions outside cells to synthesise specific products with applications in medicine, agriculture, environment and industry, particularly in the fields of drug discovery, diagnostics, biomanufacturing and...
Cell-free protein synthesis systems the best chance for your project to succeed!
Do you have a protein you want to express? Are you wondering which technology will provide you with the best results? Have you considered turning to a cell-free system? In comparison to traditional protein expression systems, cell-free protein synthesis can provide a...
Cell-free expressed OprF proteoliposomes as effective vaccine against Pseudomonas aeruginosa?
Synthelis’ cell-free liposome-based expression system was applied in the development of an OprF vaccine against Pseudomonas aeruginosa. The complete OprF outer membrane protein of P. aeruginosa was used as the immunogen and was expressed in its native form in...
Which extract is best for cell-free protein expression wheat germ or E. coli?
Cell-free protein expression offers a rapid, reliable technique for synthesis of proteins. Successfully synthesizing large quantities of high-quality product requires finding the optimal protein expression conditions, such as the type of cellular extract used. Lysate...
Five reasons to label your proteins for NMR studies using cell-free systems
Isotope labelling has been used for over 40 years to facilitate the study of protein structure using nuclear magnetic resonance (NMR) spectroscopy. During that time, traditional expression systems such as in vivo protein synthesis were the preferred options for...