Syn-Xtract™
Cell-free lysates

We are the experts in Cell-Free technology.

Packaging - Syn-Xtract - Technologie Synthelis Biotech

E. coli Cell-Free Lysates:
Take advantage of Synthelis’ proprietary technology with this reagent containing T7 RNA polymerase, enabling protein production under a T7 promoter. Simply add your reaction mix and DNA to synthesize your protein of interest.

350 μL

145 €

5 x 350 μL

510 €

Syn-Xtract™ is an open system that offers more flexibility than ready-to-use kits. Designed for experienced users, this lysate allows you to adjust any parameters you wish to test for protein production. Please note that we only provide the lysate. You will need to prepare and add the additional components (energy source, nucleotides, amino acids, etc.).

With Syn-Xtract™, you should be able to produce high-yield:
. A wide range of proteins with molecular weights up to 200 kDa,
Cytotoxic proteins (antimicrobial proteins, proteins that interfere with cell wall synthesis, etc.),
. Proteins requiring additives, such as enzymes needing heavy metals,
. Proteins that aggregate or are truncated in cellular systems,
. Membrane proteins,
. Proteins labeled with isotopic, fluorescent, or radioactive amino acids.

Application domains

Domaine

Biomedicines

Domaine

Synthetic biology

Domaine

Bioproduction

Domaine

Structural biology

Domaine

Bioeconomy

Domaine

Point-of-care bioproduction

Application notes

pdf

Validation of a phage library-based panning strategy for the selection of specific anti-C5aR monoclonal antibodies

pdf

Label-free Kinetic Analysis of Membrane Protein Interactions Using Biolayer Interferometry (BLI)

pdf

Validation of a new method to characterize proteoliposome in solution by force spectroscopy with atomic force microscopy

pdf

Successful production of antibodies against functional GPCR : Case study using functional CXCR4-containing liposomes

Our customers talk about it

Enterome logo Témoignage

"We worked with the Synthelis team for two years on a large-scale project for cell-free protein synthesis. In addition to their perfect knowledge of protein synthesis in different conditions, quantification & validation, the team is fully dedicated to our project, working with flexibility and perfect communication."

Laurent CHENE

PhD ENTEROME

Sanofi logo

"Synthelis team was very efficient to provide us with a solution to our persistent inclusion body issue. We believe that the cell-free could be a promising alternative expression system to overcome some bottlenecks."

Nicolas CHAUDET

USP Scientist SANOFI PASTEUR

AVALUN logo

"L'équipe de Synthelis a été efficace et professionnelle pour nous fournir une solution au développement rapide d’un réactif biologique pour le diagnostic de la coagulation.
La technologie acellulaire de Synthelis est particulièrement efficace pour produire en grande quantité des protéines membranaires complexes. Ce qui fait la différence dans la collaboration avec Synthelis, c'est la maitrise de leur technologie et leur professionnalisme. Je peux recommander de travailler avec Synthelis car leur équipe et leur technologie permettent d’accélérer les projets de développements."

Vincent POHER

CEO AVALUN

Logo TWIST BIOPHARMA

"At Twist Biopharma, we were looking for a supplier to produce membrane proteins as GPCRs to perform our phage display campaigns. Synthelis took our specifications for this project into consideration, adapted its cell-free expression technology, and supplied us with a challenging receptor in a very short timeline. The quality of the supplied target enabled us to successfully select antagonist and agonist VHH binders confirmed in our bioassays. We are fully satisfied with our collaboration with Synthelis."

Aaron SATO

Chief Scientific Officer TWIST BIOPHARMA

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If you encounter implementation difficulties, we will be able to accompany you.

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Cell-Free Systems Applications

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