Cell-free expressed OprF proteoliposomes as effective vaccine against Pseudomonas aeruginosa?
Synthelis’ cell-free liposome-based expression system was applied in the development of an OprF vaccine against Pseudomonas aeruginosa. The complete OprF outer membrane protein of P. aeruginosa was used as the immunogen and was expressed in its native form in...
Proteoliposomes – ideal model systems for membrane protein analysis
Proteoliposomes – ideal model systems to study the structure and function of membrane proteins in semi-native environment by cryogenic electron microscopy What are Proteoliposomes ? Proteoliposomes are lipid vesicles containing membrane proteins that can be used as...
The nucleocapsid protein of SARS-CoV-2potential target for a vaccine
What is the N-Protein ?The nucleocapsid protein (N protein) of SARS-CoV-2 is a structural protein synthesized by the virus, which plays a key role in the RNA packaging into the nucleocapsid. Its function is to mediate viral assembly through interaction with the viral...
The S-protein receptor binding domain (RBD) potential uses for SARSCoV immunization and detection
The S-protein receptor binding domain (RBD) of SARS-CoV-2 has been the subject of a thorough characterization and its importance in virus infection is well known. The potential uses of RBD of S-protein as a vaccine and as an antigen for the specific and sensitive...
Which extract is best for cell-free protein expression wheat germ or E. coli?
Cell-free protein expression offers a rapid, reliable technique for synthesis of proteins. Successfully synthesizing large quantities of high-quality product requires finding the optimal protein expression conditions, such as the type of cellular extract used. Lysate...
Five reasons to label your proteins for NMR studies using cell-free systems
Isotope labelling has been used for over 40 years to facilitate the study of protein structure using nuclear magnetic resonance (NMR) spectroscopy. During that time, traditional expression systems such as in vivo protein synthesis were the preferred options for...