Études de cas

Projet de recherche réalisé pour Sanofi Pasteur (pdf)

Solubilisation of a strongly aggregated vaccine antigen

Notes d’application


Validation of a phage library-based panning strategy for the selection of specific anti-C5aR monoclonal antibodies


Label-free Kinetic Analysis of Membrane Protein Interactions Using Biolayer Interferometry (BLI)


Validation of a new method to characterize proteoliposome in solution by force spectroscopy with atomic force microscopy


Successful production of antibodies against functional GPCR : Case study using functional CXCR4-containing liposomes


Validation of the activity of GPCRs using SPRI: case study using functional CxCR4

Publications Synthelis

OprF proteoliposome: a vaccine candidate. Géraldine Mayeux, Landry Gayet, Lavinia Liguori, Marine Odier, Donald K Martin, Sandra Cortès, Béatrice Schaack, Jean-Luc Lenormand Life Science Alliance mai 2021, 4 (6) e202000958; DOI: 10.26508/lsa.202000958

Cholesterol impacts chemokine CCR5 receptor ligand-binding activity. Pierre Calmet, Christophe Cullin, Sandra Cortès, Maylou Vang, Nada Caudy, Rim Baccouch, Jean Dessolin, Nada Taib Maamar, Sophie Lecomte, Bruno Tillier, Isabel D Alves in FEBS J. 2020

High throughput cell-free expression: From PCR product design to functionality validation. Sandra Cortès, Fatima-Ezzahra Hibti, Frydman Chiraz, Safia Ezzine in Methods and Protocols. 2019

Functional characterization of cell-free expressed Kv1.3 channel using a voltage-sensitive fluorescent dye. Sandra Cortès, Caroline Barette, Rémy Béroud, Michel De Waard, Béatrice Schaack in Elsevier. 2018

A new functional membrane protein microarray based on tethered phospholipid bilayers. Meriem Chadli, Ofelia Maniti, Christophe Marquette, Bruno Tillier, Sandra Cortès and Agnès Girard-Egrot in the Analyst. 2018

New Tethered Phospholipid Bilayers Integrating Functional G-Protein-Coupled Receptor Membrane Proteins. Meriem Chadli, Samuel Rebaud, Ofelia Maniti, Bruno Tillier, Sandra Cortès, and Agnes Girard-Egrot in Langmuir. 2017

Coupling neutron reflectivity with cell-free protein synthesis to probe membrane protein structure in supported bilayers. Thomas Soranzo, Donald K. Martin, Jean-Luc Lenormand, and Erik B. Watkins in Scientific Reports. 2017

Functional reconstitution of cell-free synthesized purified Kv channels. Stéphane Renauld , Sandra Cortès , Beate Bersch, Xavier Henry, Michel De Waard , Béatrice Schaack in Biochim Biophys Acta. 2017

Therapeutic effects of proteoliposomes on X-linked chronic granulomatous disease: proof of concept using macrophages differentiated from patient-specific induced pluripotent stem cells Julie Brault, Guillaume Vaganay, Aline Le Roy, Jean-Luc Lenormand, Sandra Cortès, Marie José Stasia in International Journal of Nanomedicine. 2017

Anti-Tumor Effects of Bak-Proteoliposomes against Glioblastoma Lavinia, Liguori, Fabio Pastorino , Xavier Rousset, Silvia Alfano, Sandra Cortès, Laura Emionite, Antonio Daga, Mirco Ponzoni and Jean-Luc Lenormand in Molecules. 2015

Functional characterization of p7 viroporin from hepatitis C virus produced in a cell-free expression system. Soranzo T, Cortès S, Gilde F, Kreir M, Picart C, Lenormand JL in Protein Expr Purif. 2015

Recombinant Nox4 cytosolic domain produced by a cell or cell-free base systems exhibits constitutive diaphorase activity. Nguyen MV, Zhang L, Lhomme S, Mouz N, Lenormand JL, Lardy B, Morel F in Biochem Biophys Res Commun. 2012 16;419(3):453-8.

Crystallization of the membrane protein hVDAC1 produced in cell-free system. Deniaud A, Liguori L, Blesneac I, Lenormand JL, Pebay-Peyroula E in Biochim Biophys Acta. 2010 -1798(8):1540-6.

Single-step production of functional OEP24 proteoliposomes. Liguori L, Blesneac I, Madern D, Vivaudou M, Lenormand JL in Protein Expr Purif. 2010 – 69,1 (106-111).

Production of recombinant proteoliposomes for therapeutic uses. Liguori L. and Lenormand JL in Methods in Enzymology, 2009 – 465 (209-223).

A bacterial cell-free expression system to produce membrane proteins and proteoliposomes: from cDNA to functional assay. Liguori L, Marques B, Lenormand JL in Curr Protoc Protein Sci. 2008. Nov;Chapter 5:Unit 5.22.

Liposomes-mediated delivery of pro-apoptotic therapeutic membrane proteins. Liguori L, Marques B, Villegas-Mendez A, Rothe R, Lenormand JL in J Control Release. 2008. 20;126(3):217-27.

Liposome-mediated cellular delivery of active gp91(phox). Marques B, Liguori L, Paclet MH, Villegas-Mendéz A, Rothe R, Morel F, Lenormand JL in PLoS One. 2007. 12;2(9):e856. PDF here.

Production of membrane proteins using cell-free expression systems. Liguori L, Marques B, Villegas-Méndez A, Rothe R, Lenormand JL in Expert Rev Proteomics. 2007. 4(1):79-90. Review.

Publications Cell-Free

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