Publications

Liposome chaperon in cell-free membrane protein synthesis: one-step preparation of KcsA-integrated liposomes and electrophysiological analysis by the planar bilayer method. Ando M, Akiyama M, Okuno D, Hirano M, Ide T, Sawada S, Sasaki T, Akiyoshi K in Biomater Sci 2015

Publications Cell-Free Synthelis Biotech

A New in Silico Antibody Similarity Measure Both Identifies Large Sets of Epitope Binders with Distinct CDRs and Accurately Predicts Off-Target Reactivity Musnier, A.; Bourquard, T.; Vallet, A.; Mathias, L.; Bruneau, G.; Ayoub, M.A.; Travert, O.; Corde, Y.; Gallay, N.; Boulo, T.; et al. . Int. J. Mol. Sci. 2022, 23, 9765. https://doi.org/10.3390/ ijms23179765

OprF proteoliposome: a vaccine candidate. Géraldine Mayeux, Landry Gayet, Lavinia Liguori, Marine Odier, Donald K Martin, Sandra Cortès, Béatrice Schaack, Jean-Luc Lenormand Life Science Alliance mai 2021, 4 (6) e202000958; DOI: 10.26508/lsa.202000958

Cholesterol impacts chemokine CCR5 receptor ligand-binding activity. Pierre Calmet, Christophe Cullin, Sandra Cortès, Maylou Vang, Nada Caudy, Rim Baccouch, Jean Dessolin, Nada Taib Maamar, Sophie Lecomte, Bruno Tillier, Isabel D Alves in FEBS J. 2020

High throughput cell-free expression: From PCR product design to functionality validation. Sandra Cortès, Fatima-Ezzahra Hibti, Frydman Chiraz, Safia Ezzine in Methods and Protocols. 2019

Functional characterization of cell-free expressed Kv1.3 channel using a voltage-sensitive fluorescent dye. Sandra Cortès, Caroline Barette, Rémy Béroud, Michel De Waard, Béatrice Schaack in Elsevier. 2018

A new functional membrane protein microarray based on tethered phospholipid bilayers. Meriem Chadli, Ofelia Maniti, Christophe Marquette, Bruno Tillier, Sandra Cortès and Agnès Girard-Egrot in the Analyst. 2018

New Tethered Phospholipid Bilayers Integrating Functional G-Protein-Coupled Receptor Membrane Proteins. Meriem Chadli, Samuel Rebaud, Ofelia Maniti, Bruno Tillier, Sandra Cortès, and Agnes Girard-Egrot in Langmuir. 2017

Coupling neutron reflectivity with cell-free protein synthesis to probe membrane protein structure in supported bilayers. Thomas Soranzo, Donald K. Martin, Jean-Luc Lenormand, and Erik B. Watkins in Scientific Reports. 2017

Functional reconstitution of cell-free synthesized purified Kv channels. Stéphane Renauld , Sandra Cortès , Beate Bersch, Xavier Henry, Michel De Waard , Béatrice Schaack in Biochim Biophys Acta. 2017

Therapeutic effects of proteoliposomes on X-linked chronic granulomatous disease: proof of concept using macrophages differentiated from patient-specific induced pluripotent stem cells Julie Brault, Guillaume Vaganay, Aline Le Roy, Jean-Luc Lenormand, Sandra Cortès, Marie José Stasia in International Journal of Nanomedicine. 2017

Anti-Tumor Effects of Bak-Proteoliposomes against Glioblastoma Lavinia, Liguori, Fabio Pastorino , Xavier Rousset, Silvia Alfano, Sandra Cortès, Laura Emionite, Antonio Daga, Mirco Ponzoni and Jean-Luc Lenormand in Molecules. 2015

Functional characterization of p7 viroporin from hepatitis C virus produced in a cell-free expression system. Soranzo T, Cortès S, Gilde F, Kreir M, Picart C, Lenormand JL in Protein Expr Purif. 2015

Recombinant Nox4 cytosolic domain produced by a cell or cell-free base systems exhibits constitutive diaphorase activity. Nguyen MV, Zhang L, Lhomme S, Mouz N, Lenormand JL, Lardy B, Morel F in Biochem Biophys Res Commun. 2012 16;419(3):453-8.

Crystallization of the membrane protein hVDAC1 produced in cell-free system. Deniaud A, Liguori L, Blesneac I, Lenormand JL, Pebay-Peyroula E in Biochim Biophys Acta. 2010 -1798(8):1540-6.

Single-step production of functional OEP24 proteoliposomes. Liguori L, Blesneac I, Madern D, Vivaudou M, Lenormand JL in Protein Expr Purif. 2010 – 69,1 (106-111).

Production of recombinant proteoliposomes for therapeutic uses. Liguori L. and Lenormand JL in Methods in Enzymology, 2009 – 465 (209-223).

A bacterial cell-free expression system to produce membrane proteins and proteoliposomes: from cDNA to functional assay. Liguori L, Marques B, Lenormand JL in Curr Protoc Protein Sci. 2008. Nov;Chapter 5:Unit 5.22.

Liposomes-mediated delivery of pro-apoptotic therapeutic membrane proteins. Liguori L, Marques B, Villegas-Mendez A, Rothe R, Lenormand JL in J Control Release. 2008. 20;126(3):217-27.

Liposome-mediated cellular delivery of active gp91(phox). Marques B, Liguori L, Paclet MH, Villegas-Mendéz A, Rothe R, Morel F, Lenormand JL in PLoS One. 2007. 12;2(9):e856. PDF here.

Production of membrane proteins using cell-free expression systems. Liguori L, Marques B, Villegas-Méndez A, Rothe R, Lenormand JL in Expert Rev Proteomics. 2007. 4(1):79-90. Review.

Chimie du substrat

A Gram-scale synthesis of luciferins derived from coelenterazine and original insights into their bioluminescence properties Coutant et al., Org. Biomol. Chem., 17, 3709 (2019)

Bioluminescence Profiling of NanoKAZ/NanoLuc Luciferase Using a Chemical Library of Coelenterazine Analogues. Coutant et al., Chem. Eur. J., 26, 948-958 (2020)

Neural engineering with photons as synaptic transmitters

Neural engineering with photons as synaptic transmitters. Porta-de-la-Riva et al., Nature Methods (2023)

Bioluminescence as a functional tool for visualizing and controlling neuronal activity in vivo. Porta-de-la-Riva et al., Neurophotonics (2024)

Analyses cellulaires

Numb-associated kinases are required for SARS-CoV-2 infection and are cellular targets for antiviral strategies. Karim et al., Antiviral Research 204,105367 (2022)

Full eradication of pre‐clinical human papilloma virus‐induced tumors by a lentiviral vaccine. Douguet et al., EMBO Molecular Medicine (2023)

Biologie  fondamentale

KCTD5 Forms Hetero-Oligomeric Complexes with Various Members of the KCTD Protein Family Liao et al. International journal of molecular sciences (2023)

Correction: Sarry et al. Host-Specific Interplay between Foot-and-Mouth Disease Virus 3D Polymerase and the Type-I Interferon Pathway. Viruses 2023, 15, 666. Sarry et al., Viruses (2023)

Pivotal role of exogenous pyruvate in human natural killer cell metabolism. Kern et al., Nature Metabolism (2025)

Bioluminescence microscopy

Volumetric imaging of fast cellular dynamics with deep learning enhanced bioluminescence microscopy Morales-Curiel et al., Communications biology (2022)

Immunoessais

A highly sensitive bioluminescent method for measuring allergen-specific IgE in microliter samples Goyard et al., Allergy (2020)

Prevalence of SARS-CoV-2 antibodies in France: results from nationwide serological surveillance Le Vu et al. Nature Communications (2021)

LuLIPLEX: A Fast, Highly Sensitive, and Multiplexed Method for the Detection of IgE Against Major Allergens Leveque et a., Allergy (2024)