{"id":5211,"date":"2025-03-22T13:56:10","date_gmt":"2025-03-22T12:56:10","guid":{"rendered":"https:\/\/synthelis.com\/?page_id=5211"},"modified":"2026-07-29T08:31:27","modified_gmt":"2026-07-29T06:31:27","slug":"syn-xpress-silver-cell-free-expression-kit","status":"publish","type":"page","link":"https:\/\/synthelis.com\/en\/syn-xpress-silver-cell-free-expression-kit\/","title":{"rendered":"Syn-Xpress\u2122 Silver Cell-Free expression kit"},"content":{"rendered":"<p>[et_pb_section fb_built=&#8221;1&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; custom_padding=&#8221;0px||0px||true|false&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_row column_structure=&#8221;3_5,2_5&#8243; use_custom_gutter=&#8221;on&#8221; gutter_width=&#8221;1&#8243; make_equal=&#8221;on&#8221; _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; width=&#8221;100%&#8221; max_width=&#8221;100%&#8221; min_height=&#8221;50vh&#8221; custom_padding=&#8221;0px||0px||false|false&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;3_5&#8243; _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; background_image=&#8221;https:\/\/synthelis.com\/wp-content\/uploads\/2024\/04\/Syn-Xpress-Synthelis.jpg&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; text_font=&#8221;PT Serif||||||||&#8221; text_text_color=&#8221;#FFFFFF&#8221; text_font_size=&#8221;16px&#8221; background_layout=&#8221;dark&#8221; min_height=&#8221;33vh&#8221; custom_margin=&#8221;0px||||false|false&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_text][\/et_pb_column][et_pb_column type=&#8221;2_5&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; use_background_color_gradient=&#8221;on&#8221; background_color_gradient_direction=&#8221;45deg&#8221; background_color_gradient_stops=&#8221;#29102e 6%|#4d1749 65%&#8221; background_color_gradient_start=&#8221;#29102e&#8221; background_color_gradient_start_position=&#8221;6%&#8221; background_color_gradient_end=&#8221;#4d1749&#8243; background_color_gradient_end_position=&#8221;65%&#8221; custom_padding=&#8221;78px|65px|78px|65px|true|true&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; text_font=&#8221;Rufina||||||||&#8221; text_text_color=&#8221;#FFFFFF&#8221; text_font_size=&#8221;4em&#8221; text_line_height=&#8221;1em&#8221; header_font=&#8221;Rufina||||||||&#8221; header_text_color=&#8221;#FFFFFF&#8221; header_font_size=&#8221;55px&#8221; header_font_size_tablet=&#8221;55px&#8221; header_font_size_phone=&#8221;40px&#8221; header_font_size_last_edited=&#8221;on|phone&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<h1>Syn-Xpress\u2122 Silver<br \/>Cell-Free<br \/>expression kit<\/h1>\n<p>[\/et_pb_text][et_pb_divider show_divider=&#8221;off&#8221; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; width=&#8221;100%&#8221; height=&#8221;50px&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_divider][et_pb_text _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; text_font=&#8221;PT Serif||||||||&#8221; text_text_color=&#8221;#FFFFFF&#8221; text_font_size=&#8221;16px&#8221; background_layout=&#8221;dark&#8221; custom_margin=&#8221;0px||||false|false&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p>Use Synthelis Cell-free Technology<br \/>to produce your proteins<\/p>\n<p>[\/et_pb_text][et_pb_divider show_divider=&#8221;off&#8221; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; width=&#8221;100%&#8221; height=&#8221;50px&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_divider][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=&#8221;1&#8243; module_id=&#8221;caracterisation&#8221; _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; background_color=&#8221;#FFFFFF&#8221; custom_margin=&#8221;0px||||false|false&#8221; custom_padding=&#8221;||||false|false&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_row column_structure=&#8221;1_2,1_2&#8243; make_equal=&#8221;on&#8221; _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; custom_margin=&#8221;||||true|false&#8221; custom_padding=&#8221;||||false|false&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;1_2&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][dnxte_coverflowslider_parent dnxte_coverflow_autoplay_delay=&#8221;3200&#8243; dnxte_coverflow_loop=&#8221;on&#8221; dnxte_coverflow_speed=&#8221;850&#8243; dnxte_coverflow_breakpoint_desktop=&#8221;1&#8243; dnxte_coverflow_arrow_color=&#8221;#29102E&#8221; dnxte_coverflow_dots_color=&#8221;#EDE8EC&#8221; dnxte_coverflow_dots_active_color=&#8221;#4D1749&#8243; _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][dnxte_coverflowslider_child admin_label_text=&#8221;Pack Syn-Xpress&#8221; coverflowslider_image=&#8221;https:\/\/synthelis.com\/wp-content\/uploads\/2026\/03\/Pack-Syn-Xpress.webp&#8221; coverflowslider_alt=&#8221;Pack Syn-Xpress&#8221; _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][\/dnxte_coverflowslider_child][dnxte_coverflowslider_child admin_label_text=&#8221;SYN-XPRESS SILVER OPEN-BOX&#8221; coverflowslider_image=&#8221;https:\/\/synthelis.com\/wp-content\/uploads\/2026\/03\/SYN-XPRESS-SILVER-OPEN-BOX-SYNTHELIS-BIOTECH-BD.webp&#8221; coverflowslider_alt=&#8221;SYN-XPRESS SILVER OPEN-BOX SYNTHELIS BIOTECH&#8221; _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][\/dnxte_coverflowslider_child][dnxte_coverflowslider_child admin_label_text=&#8221;SYN-XPRESS SILVER BAG&#8221; coverflowslider_image=&#8221;https:\/\/synthelis.com\/wp-content\/uploads\/2026\/03\/SYN-XPRESS-SILVER-BAG-SYNTHELIS-BIOTECH-BD.webp&#8221; coverflowslider_alt=&#8221;SYN-XPRESS SILVER BAG SYNTHELIS BIOTECH&#8221; _builder_version=&#8221;4.27.6&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][\/dnxte_coverflowslider_child][\/dnxte_coverflowslider_parent][\/et_pb_column][et_pb_column type=&#8221;1_2&#8243; _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; custom_css_main_element=&#8221;margin: auto;&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; text_font=&#8221;PT Serif||||||||&#8221; text_text_color=&#8221;#4D1749&#8243; text_font_size=&#8221;1.9em&#8221; text_line_height=&#8221;1.3em&#8221; text_font_size_tablet=&#8221;1.7em&#8221; text_font_size_phone=&#8221;1.6em&#8221; text_font_size_last_edited=&#8221;on|phone&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p class=\"p1\"><span class=\"s1\"><strong>Cell-free expression kit:<\/strong><br \/>With the <strong>Syn-Xpress\u2122 Silver kit<\/strong>, you have a ready-to-use reaction Mastermix\u2014just add the DNA encoding the protein you want to produce.<br \/>Simple, scalable, fast, and adaptable\u2014welcome to the limitless possibilities of Cell-Free technology with Syn-Xpress\u2122 Silver.<\/span><\/p>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=&#8221;1_5,1_5,1_5,1_5,1_5&#8243; use_custom_gutter=&#8221;on&#8221; gutter_width=&#8221;2&#8243; make_equal=&#8221;on&#8221; _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; custom_margin=&#8221;||||true|false&#8221; custom_padding=&#8221;||||false|false&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;1_5&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; text_font=&#8221;PT Serif|700|||||||&#8221; text_text_color=&#8221;#72486E&#8221; background_color=&#8221;#EDE8EC&#8221; text_orientation=&#8221;center&#8221; custom_margin=&#8221;||15px||false|false&#8221; custom_padding=&#8221;10px||10px||true|false&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p class=\"p1\">SYN90021-A5-01<br \/>5 x 50 \u03bcL<\/p>\n<p>[\/et_pb_text][et_pb_text _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; text_font=&#8221;PT Serif|700|||||||&#8221; text_text_color=&#8221;#4D1749&#8243; background_color=&#8221;#FFFFFF&#8221; text_orientation=&#8221;center&#8221; custom_margin=&#8221;0px||||false|false&#8221; custom_padding=&#8221;||||true|false&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p>175 \u20ac<\/p>\n<p>[\/et_pb_text][\/et_pb_column][et_pb_column type=&#8221;1_5&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; text_font=&#8221;PT Serif|700|||||||&#8221; text_text_color=&#8221;#72486E&#8221; background_color=&#8221;#EDE8EC&#8221; text_orientation=&#8221;center&#8221; custom_margin=&#8221;||15px||false|false&#8221; custom_padding=&#8221;10px||10px||true|false&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p class=\"p1\">SYN90021-B1-01<br \/>1 x 1 mL<\/p>\n<p>[\/et_pb_text][et_pb_text _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; text_font=&#8221;PT Serif|700|||||||&#8221; text_text_color=&#8221;#4D1749&#8243; background_color=&#8221;#FFFFFF&#8221; text_orientation=&#8221;center&#8221; custom_margin=&#8221;0px||||false|false&#8221; custom_padding=&#8221;||||true|false&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p>610 \u20ac<\/p>\n<p>[\/et_pb_text][\/et_pb_column][et_pb_column type=&#8221;1_5&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; text_font=&#8221;PT Serif|700|||||||&#8221; text_text_color=&#8221;#72486E&#8221; background_color=&#8221;#EDE8EC&#8221; text_orientation=&#8221;center&#8221; custom_margin=&#8221;||15px||false|false&#8221; custom_padding=&#8221;10px||10px||true|false&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p class=\"p1\">SYN90021-B5-01<br \/>5 x 1 mL<\/p>\n<p>[\/et_pb_text][et_pb_text _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; text_font=&#8221;PT Serif|700|||||||&#8221; text_text_color=&#8221;#4D1749&#8243; background_color=&#8221;#FFFFFF&#8221; text_orientation=&#8221;center&#8221; custom_margin=&#8221;0px||||false|false&#8221; custom_padding=&#8221;||||true|false&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p>1 920 \u20ac<\/p>\n<p>[\/et_pb_text][\/et_pb_column][et_pb_column type=&#8221;1_5&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; text_font=&#8221;PT Serif|700|||||||&#8221; text_text_color=&#8221;#72486E&#8221; background_color=&#8221;#EDE8EC&#8221; text_orientation=&#8221;center&#8221; custom_margin=&#8221;||15px||false|false&#8221; custom_padding=&#8221;10px||10px||true|false&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p class=\"p1\">SYN90021-P1-01<br \/>96 x 50 \u03bcL (1 plate)<\/p>\n<p>[\/et_pb_text][et_pb_text _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; text_font=&#8221;PT Serif|700|||||||&#8221; text_text_color=&#8221;#4D1749&#8243; background_color=&#8221;#FFFFFF&#8221; text_orientation=&#8221;center&#8221; custom_margin=&#8221;0px||||false|false&#8221; custom_padding=&#8221;||||true|false&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p>2 300 \u20ac<\/p>\n<p>[\/et_pb_text][\/et_pb_column][et_pb_column type=&#8221;1_5&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=&#8221;1&#8243; module_id=&#8221;caracterisation&#8221; _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; background_color=&#8221;#EDE8EC&#8221; custom_margin=&#8221;0px||||false|false&#8221; custom_padding=&#8221;||||false|false&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_row _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; custom_margin=&#8221;||||true|false&#8221; custom_padding=&#8221;||||false|false&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;4_4&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][dnxte_advanced_tab hover_effect=&#8221;dnxt-hover-fadeIn &#8221; tab_hover_effect=&#8221;none&#8221; tab_wrapper_margin=&#8221;||||false|false&#8221; body_wrapper_margin=&#8221;|0px||0px|false|false&#8221; body_wrapper_padding=&#8221;30px|0px||0px|false|false&#8221; tab_container_bg_color=&#8221;RGBA(255,255,255,0)&#8221; tab_single_bg_color=&#8221;RGBA(255,255,255,0)&#8221; tab_active_bg_color=&#8221;RGBA(255,255,255,0)&#8221; content_container_bg_color=&#8221;RGBA(255,255,255,0)&#8221; _builder_version=&#8221;4.27.7&#8243; _module_preset=&#8221;default&#8221; tab_title_font=&#8221;PT Serif|700|||||||&#8221; tab_title_text_color=&#8221;#D2C6D2&#8243; tab_title_font_size=&#8221;25px&#8221; tab_active_title_text_color=&#8221;#4D1749&#8243; width=&#8221;100%&#8221; max_width=&#8221;100%&#8221; custom_margin=&#8221;0px|0px||0px|false|false&#8221; custom_padding=&#8221;|0px||0px|false|false&#8221; hover_enabled=&#8221;0&#8243; border_width_bottom_tab_active=&#8221;3px&#8221; border_color_bottom_tab_active=&#8221;#4D1749&#8243; global_colors_info=&#8221;{}&#8221; sticky_enabled=&#8221;0&#8243;][dnxte_advanced_tab_item admin_label_text=&#8221;Descriptif&#8221; tab_title=&#8221;Description&#8221; body_description=&#8221;<\/p>\n<p>The Syn-Xpress\u2122 Silver kit is designed for users seeking a simple, fast, and reliable solution to produce their protein of interest using cell-free protein synthesis (CFPS). It contains the cell lysate along with a complete energy mix (nucleotides, energy source, amino acids, salts, and cofactors), pre-formulated to ensure optimal performance.<\/p>\n<p>With Syn-Xpress\u2122 Silver, you only need to add your expression DNA (plasmid or linear) and, if necessary, specific additives (cofactors, chaperones, ions, etc.) depending on the requirements of your target protein. This configuration provides s<strong>ignificant time savings while ensuring high reproducibility.<\/strong><\/p>\n<p>Each Syn-Xpress\u2122 Silver kit has a <strong>control plasmid encoding Syn-GFP<\/strong>, a GFP optimized for cell-free expression. This control allows you to validate your experimental conditions.<\/p>\n<p><strong>The Syn-Xpress\u2122 Silver kit includes:<\/strong><br \/>. The Mastermix containing the lysate as well as the complete energy mix.<br \/>. One tube of control plasmid encoding Syn-GFP<br \/>. The user manual<\/p>\n<p><em>Notes : <\/em><br \/><em>The 96 x 50\u202f\u00b5L format is provided directly in a 96-well plate, while the other formats are supplied in tubes.<\/em><br data-start=%22122%22 data-end=%22125%22 \/><em>The kits are composed of 80% Mastermix, leaving you with 20% of the final reaction volume to add your DNA, any potential additives, and nuclease-free water to reach the desired volume. For example, the 50\u202f\u00b5L kits contain 40\u202f\u00b5L of Mastermix, and the 1\u202fmL kits contain 800\u202f\u00b5L of Mastermix.<\/em><\/p>\n<p>Custom formats are available upon request, enabling high-throughput screening or scale-up. When scaling up, the system\u2019s yields remain linear.<\/p>\n<p>&#8221; tab_item_margin=&#8221;||||false|false&#8221; _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; description_font=&#8221;PT Serif||||||||&#8221; description_font_size=&#8221;1.4em&#8221; description_line_height=&#8221;1.7em&#8221; width=&#8221;1080px&#8221; max_width=&#8221;100%&#8221; global_colors_info=&#8221;{}&#8221;][\/dnxte_advanced_tab_item][dnxte_advanced_tab_item admin_label_text=&#8221;Avantages&#8221; tab_title=&#8221;Advantages&#8221; body_description=&#8221;<\/p>\n<p>With Syn-Xpress\u2122 Silver, produce your own proteins:<br \/>.<strong> easily,<\/strong> by simply adding DNA,<br \/>. <strong>quickly<\/strong>, in just a few hours,<br \/>. <strong>with a wide variety<\/strong> : soluble, hard-to-express, cytotoxic, membrane proteins, enzymes, antigens, etc.,<br \/>. <strong>using an open system<\/strong> that allows the addition of various additives,<br \/>. <strong>with isotopic labeling or incorporation of non-natural amino acids,<\/strong><br \/>. <strong>by screening a large number of mutants,<\/strong><br \/>. <strong>for numerous applications<\/strong>: functional studies, drug discovery, enzyme engineering, synthetic biology, and more.<\/p>\n<p>&#8221; 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global_colors_info=&#8221;{}&#8221;][et_pb_accordion open_toggle_text_color=&#8221;#4D1749&#8243; open_toggle_background_color=&#8221;RGBA(255,255,255,0)&#8221; closed_toggle_background_color=&#8221;RGBA(255,255,255,0)&#8221; toggle_icon=&#8221;&#xe044;||divi||400&#8243; icon_color=&#8221;#4D1749&#8243; use_icon_font_size=&#8221;on&#8221; icon_font_size=&#8221;24px&#8221; _builder_version=&#8221;4.27.7&#8243; _module_preset=&#8221;default&#8221; toggle_text_color=&#8221;#4D1749&#8243; toggle_font=&#8221;PT Serif|700|||||||&#8221; toggle_font_size=&#8221;24px&#8221; body_font=&#8221;PT Serif||||||||&#8221; body_font_size=&#8221;1.4em&#8221; body_line_height=&#8221;1.4em&#8221; background_color=&#8221;RGBA(255,255,255,0)&#8221; border_width_all=&#8221;0px&#8221; border_style_all=&#8221;solid&#8221; border_width_bottom=&#8221;1px&#8221; border_color_bottom=&#8221;#4D1749&#8243; global_colors_info=&#8221;{}&#8221; toggle_icon__hover_enabled=&#8221;off|desktop&#8221; toggle_icon__hover=&#8221;\ue044||divi||400&#8243;][et_pb_accordion_item title=&#8221;What is cell-free protein expression (CFPS)?&#8221; open=&#8221;on&#8221; _builder_version=&#8221;4.27.7&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p>Cell-free protein synthesis (CFPS), also known as cell-free protein expression, is a protein production method performed outside living cells. It uses cellular extracts containing the natural transcription and translation machinery (enzymes, tRNAs, ribosomes, etc.), supplemented with an energy source, nucleoside triphosphates (NTPs), amino acids, and other additives. Starting from DNA or RNA, the protein of interest is produced directly in vitro. This approach reproduces gene expression without the need for a living cellular unit.<\/p>\n<p>[\/et_pb_accordion_item][et_pb_accordion_item title=&#8221;How does a cell-free protein expression system work?&#8221; _builder_version=&#8221;4.27.7&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221; open=&#8221;off&#8221;]<\/p>\n<p>A genetic template (DNA or RNA) is added to an active cell lysate containing the components required for transcription and translation. When starting from DNA (plasmid or linear), messenger RNA is first synthesized, then ribosomes assemble the polypeptide chain. The reaction takes place in an open and controlled environment. Biochemical conditions can be adjusted during synthesis.<\/p>\n<p>[\/et_pb_accordion_item][et_pb_accordion_item title=&#8221;What are the advantages of cell-free systems compared to cell-based systems?&#8221; _builder_version=&#8221;4.27.7&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221; open=&#8221;off&#8221;]<\/p>\n<p>Cell-free systems provide rapid results without the need for cell line construction or prolonged culture. The open system allows direct adjustment of the synthesis and folding environment, as well as the addition of cofactors, ligands, or other additives. It also reduces expression failures linked to cytotoxicity, instability, or aggregation. Cell-free expression is particularly well suited for early-stage characterization and experimental validation.<\/p>\n<p>[\/et_pb_accordion_item][et_pb_accordion_item title=&#8221;What types of recombinant proteins can be expressed using cell-free systems?&#8221; _builder_version=&#8221;4.27.7&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221; open=&#8221;off&#8221;]<\/p>\n<p>Any type of protein can be expressed using cell-free systems, including membrane proteins through the addition of stabilizers (lipids or detergents), multi-subunit protein complexes through co-translation of several polypeptides, and toxic proteins.<\/p>\n<p>[\/et_pb_accordion_item][et_pb_accordion_item title=&#8221;When should cell-free protein synthesis (CFPS) be chosen?&#8221; _builder_version=&#8221;4.27.7&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221; open=&#8221;off&#8221;]<\/p>\n<p>CFPS is particularly relevant when a protein is difficult to express or when rapid results are required. It is used for target validation, interaction studies, labeling, prototyping, and variant screening. It enables the rapid production of a testable protein before considering larger-scale production either in cell-free or cell-based systems.<\/p>\n<p>[\/et_pb_accordion_item][\/et_pb_accordion][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=&#8221;1&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; background_color=&#8221;#FFFFFF&#8221; background_color_gradient_direction=&#8221;90deg&#8221; background_color_gradient_start=&#8221;#eeeff3&#8243; background_color_gradient_end=&#8221;#aaaaaa&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_row column_structure=&#8221;1_2,1_2&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; custom_margin=&#8221;||0px||false|false&#8221; custom_padding=&#8221;||0px||false|false&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;1_2&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; header_font=&#8221;PT Serif|700|||||||&#8221; header_text_color=&#8221;#4D1749&#8243; header_4_font=&#8221;PT Serif|700|||||||&#8221; header_4_font_size=&#8221;30px&#8221; border_color_bottom=&#8221;#EEEFF3&#8243; global_colors_info=&#8221;{}&#8221;]<\/p>\n<h4>Our customers talk about it<\/h4>\n<p>[\/et_pb_text][\/et_pb_column][et_pb_column type=&#8221;1_2&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=&#8221;1_5,3_5,1_5&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;1_5&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_column][et_pb_column type=&#8221;3_5&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][dnxte_testimonial_parent dnxte_testimonial_use_icon=&#8221;on&#8221; dnxt_testimonial_autoplay_delay=&#8221;4000&#8243; dnxt_testimonial_loop=&#8221;on&#8221; dnxt_testimonial_speed=&#8221;1000&#8243; dnxt_testimonial_dots_active_color=&#8221;#4D1749&#8243; dnxt_testimonial_pause_on_hover=&#8221;on&#8221; dnxt_testimonial_rating_color=&#8221;#4D1749&#8243; dnxt_testimonial_centered_slides=&#8221;on&#8221; dnxt_testimonial_image_width=&#8221;100px&#8221; _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; header_text_align=&#8221;center&#8221; global_colors_info=&#8221;{}&#8221;][dnxte_testimonial_child admin_label_text=&#8221;Laurent CHENE&#8221; dnxte_testimonial_name=&#8221;Laurent CHENE&#8221; dnxte_testimonial_position=&#8221;PhD&#8221; company_name=&#8221;ENTEROME&#8221; company_link=&#8221;https:\/\/www.enterome.com\/&#8221; company_link_new_window=&#8221;on&#8221; dnxte_testimonial_description=&#8221;<\/p>\n<p class=%22p1%22><img src=%22https:\/\/synthelis.com\/wp-content\/uploads\/2022\/03\/Synthelis-Temoignages-Enterome.jpg%22 width=%22300%22 height=%2291%22 alt=%22Enterome logo T\u00e9moignage%22 class=%22wp-image-1044 alignnone size-full%22 style=%22display: block; margin-left: auto; margin-right: auto;%22 \/><\/p>\n<p class=%22p1%22>%22We worked with the Synthelis team for two years on a large-scale project for cell-free protein synthesis. In addition to their perfect knowledge of protein synthesis in different conditions, quantification &#038; validation, the team is fully dedicated to our project, working with flexibility and perfect communication.%22<\/p>\n<p>&#8221; dnxte_testimonial_logo_alt=&#8221;Laurent Chene, PhD Enterome &#8221; description_bg_color=&#8221;RGBA(255,255,255,0)&#8221; _builder_version=&#8221;4.19.4&#8243; _module_preset=&#8221;default&#8221; header_font=&#8221;PT Serif|700|||||||&#8221; header_text_align=&#8221;center&#8221; header_text_color=&#8221;#4D1749&#8243; position_font=&#8221;PT Serif||||||||&#8221; position_text_align=&#8221;center&#8221; company_name_font=&#8221;PT Serif|700|||||||&#8221; description_font=&#8221;Rufina||||||||&#8221; description_text_color=&#8221;#000000&#8243; description_font_size=&#8221;22px&#8221; description_quote_border_weight=&#8221;4px&#8221; description_quote_font=&#8221;Rufina||||||||&#8221; description_quote_text_color=&#8221;#EDE8EC&#8221; description_quote_font_size=&#8221;13px&#8221; global_colors_info=&#8221;{}&#8221;][\/dnxte_testimonial_child][dnxte_testimonial_child admin_label_text=&#8221;Nicolas CHAUDET&#8221; dnxte_testimonial_name=&#8221;Nicolas CHAUDET&#8221; dnxte_testimonial_position=&#8221;USP Scientist&#8221; company_name=&#8221;SANOFI PASTEUR&#8221; company_link=&#8221;https:\/\/www.sanofi.fr\/&#8221; company_link_new_window=&#8221;on&#8221; dnxte_testimonial_description=&#8221;<\/p>\n<p class=%22p1%22 style=%22text-align: center;%22><img src=%22https:\/\/synthelis.com\/wp-content\/uploads\/2022\/03\/Synthelis-Temoignages-Sanofi.jpg%22 width=%22300%22 height=%2291%22 alt=%22Sanofi logo%22 class=%22wp-image-1803 alignnone size-full%22 \/><\/p>\n<p class=%22p1%22 style=%22text-align: center;%22>%22Synthelis team was very efficient to provide us with a solution to our persistent inclusion body issue. We believe that the cell-free could be a promising alternative expression system to overcome some bottlenecks.%22<\/p>\n<p>&#8221; dnxte_testimonial_logo_alt=&#8221;Laurent Chene, PhD Enterome &#8221; _builder_version=&#8221;4.19.4&#8243; _module_preset=&#8221;default&#8221; header_font=&#8221;PT Serif|700|||||||&#8221; header_text_color=&#8221;#4D1749&#8243; position_font=&#8221;PT Serif||||||||&#8221; company_name_font=&#8221;PT Serif|700|||||||&#8221; description_font=&#8221;Rufina||||||||&#8221; description_text_color=&#8221;#000000&#8243; description_font_size=&#8221;22px&#8221; description_quote_border_weight=&#8221;4px&#8221; description_quote_font=&#8221;Rufina||||||||&#8221; description_quote_text_color=&#8221;#EDE8EC&#8221; description_quote_font_size=&#8221;13px&#8221; global_colors_info=&#8221;{}&#8221;][\/dnxte_testimonial_child][dnxte_testimonial_child admin_label_text=&#8221;Vincent POHER &#8221; dnxte_testimonial_name=&#8221;Vincent POHER &#8221; dnxte_testimonial_position=&#8221;CEO&#8221; company_name=&#8221;AVALUN&#8221; company_link=&#8221;https:\/\/avalun.com\/&#8221; company_link_new_window=&#8221;on&#8221; dnxte_testimonial_description=&#8221;<\/p>\n<p class=%22p1%22 style=%22text-align: center;%22><img src=%22https:\/\/synthelis.com\/wp-content\/uploads\/2022\/04\/Synthelis-Temoignages-Avalun.jpg%22 width=%22300%22 height=%2291%22 alt=%22AVALUN logo%22 class=%22wp-image-2631 alignnone size-full%22 \/><\/p>\n<p class=%22p1%22 style=%22text-align: center;%22>%22Synthelis&#8217; team was efficient and professional in providing us with a solution for the rapid development of a biological reagent for coagulation diagnosis. Synthelis&#8217; cell-free technology is particularly effective in producing large quantities of complex membrane proteins. What makes the difference in working with Synthelis is their expertise in their technology and their professionalism. I can recommend working with Synthelis because their team and their technology allow to accelerate project development.%22<\/p>\n<p>&#8221; dnxte_testimonial_logo_alt=&#8221;Laurent Chene, PhD Enterome &#8221; _builder_version=&#8221;4.27.4&#8243; _module_preset=&#8221;default&#8221; header_font=&#8221;PT Serif|700|||||||&#8221; header_text_color=&#8221;#4D1749&#8243; position_font=&#8221;PT Serif||||||||&#8221; company_name_font=&#8221;PT Serif|700|||||||&#8221; company_name_text_align=&#8221;center&#8221; description_font=&#8221;Rufina||||||||&#8221; description_text_color=&#8221;#000000&#8243; description_font_size=&#8221;22px&#8221; description_quote_border_weight=&#8221;4px&#8221; description_quote_font=&#8221;Rufina||||||||&#8221; description_quote_text_color=&#8221;#EDE8EC&#8221; description_quote_font_size=&#8221;13px&#8221; global_colors_info=&#8221;{}&#8221;][\/dnxte_testimonial_child][dnxte_testimonial_child admin_label_text=&#8221;Aaron SATO &#8221; dnxte_testimonial_name=&#8221;Aaron SATO &#8221; dnxte_testimonial_position=&#8221;Chief Scientific Officer &#8221; company_name=&#8221;TWIST BIOPHARMA&#8221; company_link=&#8221;https:\/\/www.twistbioscience.com\/&#8221; company_link_new_window=&#8221;on&#8221; dnxte_testimonial_description=&#8221;<\/p>\n<p class=%22p1%22 style=%22text-align: center;%22><img src=%22https:\/\/synthelis.com\/wp-content\/uploads\/2023\/03\/Synthelis-Temoignages-Twist.jpg%22 width=%22300%22 height=%2291%22 alt=%22Logo TWIST BIOPHARMA%22 class=%22wp-image-3028 alignnone size-full%22 \/><\/p>\n<p class=%22p1%22 style=%22text-align: center;%22>%22At Twist Biopharma, we were looking for a supplier to produce membrane proteins as GPCRs to perform our phage display campaigns. Synthelis took our specifications for this project into consideration, adapted its cell-free expression technology, and supplied us with a challenging receptor in a very short timeline. The quality of the supplied target enabled us to successfully select antagonist and agonist VHH binders confirmed in our bioassays. We are fully satisfied with our collaboration with Synthelis.%22<\/p>\n<p>&#8221; dnxte_testimonial_logo_alt=&#8221;Laurent Chene, PhD Enterome &#8221; _builder_version=&#8221;4.19.4&#8243; _module_preset=&#8221;default&#8221; header_font=&#8221;PT Serif|700|||||||&#8221; header_text_color=&#8221;#4D1749&#8243; position_font=&#8221;PT Serif||||||||&#8221; company_name_font=&#8221;PT Serif|700|||||||&#8221; company_name_text_align=&#8221;center&#8221; description_font=&#8221;Rufina||||||||&#8221; description_text_color=&#8221;#000000&#8243; description_font_size=&#8221;22px&#8221; description_quote_border_weight=&#8221;4px&#8221; description_quote_font=&#8221;Rufina||||||||&#8221; description_quote_text_color=&#8221;#EDE8EC&#8221; description_quote_font_size=&#8221;13px&#8221; global_colors_info=&#8221;{}&#8221;][\/dnxte_testimonial_child][\/dnxte_testimonial_parent][\/et_pb_column][et_pb_column type=&#8221;1_5&#8243; _builder_version=&#8221;4.16&#8243; 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global_colors_info=&#8221;{}&#8221;][\/dnxte_coverflowslider_child][dnxte_coverflowslider_child admin_label_text=&#8221;Bioluminescence Pro-substrate Hikarazine\u2122 Z108&#8243; coverflowslider_layouts=&#8221;below-image&#8221; coverflowslider_text=&#8221;Bioluminescence Pro-substrate Hikarazine\u2122 Z108&#8243; coverflowslider_image=&#8221;https:\/\/synthelis.com\/wp-content\/uploads\/2025\/03\/Hikarazine-Boite-108.webp&#8221; dnxte_coverflow_button_link=&#8221;https:\/\/synthelis.com\/en\/pro-substrat-bioluminescent-hikarazine-z-108\/&#8221; coverflow_hover_overlay_color=&#8221;rgba(114,72,110,0.1)&#8221; _builder_version=&#8221;4.27.4&#8243; _dynamic_attributes=&#8221;link_option_url&#8221; _module_preset=&#8221;default&#8221; header_font=&#8221;PT Serif|700|||||||&#8221; header_text_color=&#8221;#72486E&#8221; link_option_url=&#8221;@ET-DC@eyJkeW5hbWljIjp0cnVlLCJjb250ZW50IjoicG9zdF9saW5rX3VybF9wYWdlIiwic2V0dGluZ3MiOnsicG9zdF9pZCI6IjUyMjUifX0=@&#8221; 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show_divider=&#8221;off&#8221; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; width=&#8221;100%&#8221; height=&#8221;50px&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_divider][et_pb_text _builder_version=&#8221;4.18.0&#8243; _module_preset=&#8221;default&#8221; text_font=&#8221;PT Serif||||||||&#8221; text_text_color=&#8221;#FFFFFF&#8221; text_font_size=&#8221;16px&#8221; background_layout=&#8221;dark&#8221; custom_margin=&#8221;0px||||false|false&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p class=\"p1\">If you encounter implementation difficulties, we will be able to accompany you.<\/p>\n<p>[\/et_pb_text][et_pb_divider show_divider=&#8221;off&#8221; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; width=&#8221;100%&#8221; height=&#8221;50px&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_divider][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=&#8221;1&#8243; _builder_version=&#8221;4.18.0&#8243; 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width=&#8221;100%&#8221; max_width=&#8221;100%&#8221; min_height=&#8221;70vh&#8221; custom_padding=&#8221;0px||0px||false|false&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;1_3&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; background_image=&#8221;https:\/\/synthelis.com\/wp-content\/uploads\/2022\/01\/Synthelis-MemeUneProteineABesoindExprimerSonPotentiel-Part1.jpg&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; text_font=&#8221;PT Serif||||||||&#8221; text_text_color=&#8221;#FFFFFF&#8221; text_font_size=&#8221;16px&#8221; background_layout=&#8221;dark&#8221; min_height=&#8221;50vh&#8221; min_height_tablet=&#8221;50vh&#8221; min_height_phone=&#8221;50vh&#8221; min_height_last_edited=&#8221;on|phone&#8221; custom_margin=&#8221;0px||||false|false&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_text][\/et_pb_column][et_pb_column type=&#8221;1_3&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; use_background_color_gradient=&#8221;on&#8221; background_color_gradient_direction=&#8221;45deg&#8221; background_color_gradient_stops=&#8221;#29102e 6%|#4d1749 65%&#8221; background_color_gradient_start=&#8221;#29102e&#8221; background_color_gradient_start_position=&#8221;6%&#8221; background_color_gradient_end=&#8221;#4d1749&#8243; background_color_gradient_end_position=&#8221;65%&#8221; custom_padding=&#8221;78px|65px|78px|65px|true|true&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; text_font=&#8221;Rufina||||||||&#8221; text_text_color=&#8221;#FFFFFF&#8221; text_font_size=&#8221;55px&#8221; text_line_height=&#8221;1em&#8221; text_font_size_tablet=&#8221;55px&#8221; text_font_size_phone=&#8221;40px&#8221; text_font_size_last_edited=&#8221;on|phone&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p>Even<br \/>a protein<br \/>needs<br \/>to express<br \/>its potential.<\/p>\n<p>[\/et_pb_text][et_pb_divider show_divider=&#8221;off&#8221; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; width=&#8221;100%&#8221; height=&#8221;50px&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_divider][et_pb_text _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; text_font=&#8221;PT Serif||||||||&#8221; text_text_color=&#8221;#FFFFFF&#8221; text_font_size=&#8221;16px&#8221; background_layout=&#8221;dark&#8221; custom_margin=&#8221;0px||||false|false&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p>Cell-Free Systems Applications<\/p>\n<p>[\/et_pb_text][\/et_pb_column][et_pb_column type=&#8221;1_3&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; background_image=&#8221;https:\/\/synthelis.com\/wp-content\/uploads\/2022\/01\/Synthelis-MemeUneProteineABesoindExprimerSonPotentiel-Part2.jpg&#8221; 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decoding=\"async\" width=\"660\" height=\"440\" src=\"https:\/\/synthelis.com\/wp-content\/uploads\/2026\/03\/SYN-XPRESS-SILVER-OPEN-BOX-SYNTHELIS-BIOTECH-BD.webp\" alt=\"SYN-XPRESS SILVER OPEN-BOX SYNTHELIS BIOTECH\" title=\"\" srcset=\"https:\/\/synthelis.com\/wp-content\/uploads\/2026\/03\/SYN-XPRESS-SILVER-OPEN-BOX-SYNTHELIS-BIOTECH-BD.webp 660w, https:\/\/synthelis.com\/wp-content\/uploads\/2026\/03\/SYN-XPRESS-SILVER-OPEN-BOX-SYNTHELIS-BIOTECH-BD-480x320.webp 480w\" sizes=\"(min-width: 0px) and (max-width: 480px) 480px, (min-width: 481px) 660px, 100vw\" class=\"wp-image-7323 img-fluid\" \/>\n                <\/span>\n            <\/div>\n            <div class=\"dnxte-coverflow-multitext \">\n                \n                \n                \n            <\/div>\n\t\t\t\t<\/div>\n\t\t\t<\/div><div class=\"et_pb_module dnxte_coverflowslider_child dnxte_coverflowslider_child_2\">\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t\n\t\t\t\t<div class=\"et_pb_module_inner\">\n\t\t\t\t\t<div class=\"dnxte-coverflow-image-container\" data-image-position=\"\">\n                <span href=\"https:\/\/synthelis.com\/wp-content\/uploads\/2026\/03\/SYN-XPRESS-SILVER-BAG-SYNTHELIS-BIOTECH-BD.webp\" class=\"dnxte-coverflow-image-link\" data-title=\"\" data-link=\",_self\">\n                    <div class=\"dnxte-coverflow-overlay-color\"><\/div>\n                    <div class=\"dnxte-coverflow-overlay-color-hover\"><\/div>\n                    <img loading=\"lazy\" decoding=\"async\" width=\"660\" height=\"440\" src=\"https:\/\/synthelis.com\/wp-content\/uploads\/2026\/03\/SYN-XPRESS-SILVER-BAG-SYNTHELIS-BIOTECH-BD.webp\" alt=\"SYN-XPRESS SILVER BAG SYNTHELIS BIOTECH\" title=\"\" srcset=\"https:\/\/synthelis.com\/wp-content\/uploads\/2026\/03\/SYN-XPRESS-SILVER-BAG-SYNTHELIS-BIOTECH-BD.webp 660w, https:\/\/synthelis.com\/wp-content\/uploads\/2026\/03\/SYN-XPRESS-SILVER-BAG-SYNTHELIS-BIOTECH-BD-480x320.webp 480w\" sizes=\"(min-width: 0px) and (max-width: 480px) 480px, (min-width: 481px) 660px, 100vw\" class=\"wp-image-7321 img-fluid\" \/>\n                <\/span>\n            <\/div>\n            <div class=\"dnxte-coverflow-multitext \">\n                \n                \n                \n            <\/div>\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n                    <\/div>\n                    <div class=\"swiper-pagination swiper-pagination-clickable swiper-pagination-bullets swiper-pagination-bullets-dynamic mt-10\"><\/div>\n                <\/div>\n                \n            <\/div>\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t<\/div>Cell-free expression kit:With the Syn-Xpress\u2122 [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","_surecart_dashboard_logo_width":"180px","_surecart_dashboard_show_logo":true,"_surecart_dashboard_navigation_orders":true,"_surecart_dashboard_navigation_invoices":true,"_surecart_dashboard_navigation_subscriptions":true,"_surecart_dashboard_navigation_downloads":true,"_surecart_dashboard_navigation_billing":true,"_surecart_dashboard_navigation_account":true,"footnotes":""},"dipi_cpt_category":[],"folder":[49],"class_list":["post-5211","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.2 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Syn-Xpress\u2122 Silver Cell-Free expression kit - Synthelis<\/title>\n<meta name=\"description\" content=\"Syn-Xpress\u2122: fast, 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