[et_pb_section fb_built=”1″ _builder_version=”4.16″ _module_preset=”default” use_background_color_gradient=”on” background_color_gradient_direction=”45deg” background_color_gradient_stops=”#29102e 6%|#4d1749 65%” background_color_gradient_start=”#29102e” background_color_gradient_start_position=”6%” background_color_gradient_end=”#4d1749″ background_color_gradient_end_position=”65%” custom_padding=”0px||0px||true|false” global_colors_info=”{}”][et_pb_row use_custom_gutter=”on” gutter_width=”1″ make_equal=”on” _builder_version=”4.16″ _module_preset=”default” custom_padding=”0px||0px||false|false” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” background_color_gradient_direction=”45deg” background_color_gradient_start=”#29102e” background_color_gradient_end=”#4d1749″ custom_padding=”78px||78px||true|true” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” text_font=”Rufina||||||||” text_text_color=”#FFFFFF” text_font_size=”4em” text_line_height=”1em” header_font=”Rufina||||||||” header_text_color=”#FFFFFF” header_font_size=”55px” header_font_size_tablet=”55px” header_font_size_phone=”40px” header_font_size_last_edited=”on|phone” global_colors_info=”{}”]<\/p>\n
[\/et_pb_text][et_pb_divider show_divider=”off” _builder_version=”4.16″ _module_preset=”default” width=”100%” height=”50px” global_colors_info=”{}”][\/et_pb_divider][dnxte_button button_text=”Bioluminescence” button_link=”https:\/\/synthelis.com\/?page_id=4730\/#faqbio” btn_icon=”$||divi||400″ btn_icon_color=”#FFFFFF” btn_icon_placement=”left” dnxt_hover_2d=”dnxt-hover-backward” _builder_version=”4.27.4″ _module_preset=”default” btn_fonts_font=”Rufina||||||||” btn_fonts_text_color=”#D2C6D2″ btn_fonts_font_size=”24px” custom_margin=”0px||0px||true|false” custom_padding=”0px||0px||false|false” border_width_all_btn_border=”0px” border_color_all_btn_border=”RGBA(255,255,255,0)” border_color_top_btn_border=”#D2C6D2″ border_color_bottom_btn_border=”#D2C6D2″ global_colors_info=”{}”][\/dnxte_button][et_pb_divider show_divider=”off” _builder_version=”4.16″ _module_preset=”default” width=”100%” height=”50px” global_colors_info=”{}”][\/et_pb_divider][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ module_id=”faqbio” _builder_version=”4.27.4″ _module_preset=”default” background_color=”#EDE8EC” custom_padding=”||80px||false|false” global_colors_info=”{}”][et_pb_row use_custom_gutter=”on” gutter_width=”1″ make_equal=”on” _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” header_font=”PT Serif|700|||||||” header_text_color=”#4D1749″ header_3_font=”PT Serif|700|||||||” header_3_font_size=”30px” custom_margin=”||5px||false|false” custom_padding=”||||false|false” border_color_bottom=”#EEEFF3″ global_colors_info=”{}”]<\/p>\n
[\/et_pb_text][et_pb_divider color=”#D2C6D2″ divider_position=”center” _builder_version=”4.18.0″ _module_preset=”default” custom_margin=”||20px||false|false” global_colors_info=”{}”][\/et_pb_divider][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”||||true|false” custom_padding=”0px||||false|false” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_accordion open_toggle_text_color=”#4D1749″ open_toggle_background_color=”RGBA(255,255,255,0)” closed_toggle_background_color=”RGBA(255,255,255,0)” toggle_icon=”||divi||400″ icon_color=”#4D1749″ use_icon_font_size=”on” icon_font_size=”24px” _builder_version=”4.27.4″ _module_preset=”default” toggle_text_color=”#4D1749″ toggle_font=”PT Serif|700|||||||” toggle_font_size=”24px” body_font=”PT Serif||||||||” body_font_size=”1.4em” body_line_height=”1.4em” background_color=”RGBA(255,255,255,0)” hover_enabled=”0″ border_width_all=”0px” border_style_all=”solid” border_width_bottom=”1px” border_color_bottom=”#4D1749″ global_colors_info=”{}” toggle_icon__hover_enabled=”off|desktop” toggle_icon__hover=”\ue044||divi||400″ sticky_enabled=”0″][et_pb_accordion_item title=”Which luciferases are compatible with Hikarazine\u2122?” open=”on” _builder_version=”4.27.4″ _module_preset=”default” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″]<\/p>\n
Hikarazine\u2122 has been specifically designed for use with luciferase enzymes derived from Oplophorus gracilirostris<\/i>.<\/p>\n
[\/et_pb_accordion_item][et_pb_accordion_item title=”Which Hikarazine\u2122 should I use?” _builder_version=”4.27.4″ _module_preset=”default” hover_enabled=”0″ global_colors_info=”{}” open=”off” sticky_enabled=”0″]<\/p>\n
The choice between Hikarazine\u2122 Z108 and Hikarazine\u2122 Z103 depends on your specific assay.<\/p>\n
[\/et_pb_accordion_item][et_pb_accordion_item title=”Which buffer should I use with Hikarazine\u2122?” _builder_version=”4.27.4″ _module_preset=”default” hover_enabled=”0″ global_colors_info=”{}” open=”off” sticky_enabled=”0″]<\/p>\n
The choice of buffer is crucial for optimal bioluminescence. PBS is recommended, but other buffers may be used depending on the assay. For instance, adding 0.01% Tween can help with complex matrices, though it may slightly increase background noise. Additionally, varying cation compositions can influence both brightness and bioluminescence half-life. If you need further guidance after testing in your specific assay, please contact our experts.<\/p>\n
[\/et_pb_accordion_item][et_pb_accordion_item title=”What factors influence the light emission process and brightness?” _builder_version=”4.27.4″ _module_preset=”default” hover_enabled=”0″ global_colors_info=”{}” open=”off” sticky_enabled=”0″]<\/p>\n
The light emission and brightness of Hikarazine\u2122 are influenced by several factors:<\/p>\n
These factors must be optimized to ensure the best performance of the bioluminescent system using Hikarazine\u2122.<\/p>\n
[\/et_pb_accordion_item][et_pb_accordion_item title=”What are the optimal storage conditions for maximum stability of the pro-substrate?” _builder_version=”4.27.4″ _module_preset=”default” hover_enabled=”0″ global_colors_info=”{}” open=”off” sticky_enabled=”0″]<\/p>\n
To ensure stability for up to two years, store the vials at room temperature, away from heat, light, and humidity.<\/p>\n
[\/et_pb_accordion_item][et_pb_accordion_item title=”Why is the pro-substrate powder darkening?” _builder_version=”4.27.4″ _module_preset=”default” hover_enabled=”0″ global_colors_info=”{}” open=”off” sticky_enabled=”0″]<\/p>\n
A slight darkening of the surface of the solid may occur over time. However, this does not affect the bioluminescence signal, provided the pro-substrate is used under standard conditions recommended in the IFU.<\/p>\n
[\/et_pb_accordion_item][et_pb_accordion_item title=”What are the optimal storage conditions for the stock solution?” _builder_version=”4.27.4″ _module_preset=”default” hover_enabled=”0″ global_colors_info=”{}” open=”off” sticky_enabled=”0″]<\/p>\n
The stock solution is acidic, which enhances its stability. When stored at 4\u00b0C, it remains stable for 6 months. For extended stability, it can be stored at -20\u00b0C, with ongoing tests to determine the exact duration.<\/p>\n
[\/et_pb_accordion_item][et_pb_accordion_item title=”What is the peak emission of Hikarazine\u2122?” _builder_version=”4.27.4″ _module_preset=”default” hover_enabled=”0″ global_colors_info=”{}” open=”off” sticky_enabled=”0″]<\/p>\n
The peak bioluminescence emission of activated luciferins Hikarazine\u2122 Z103 and Z108 has been measured at 450 \u00b1 3 nm.<\/span><\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”What are the recommendations for small animal imaging?” _builder_version=”4.27.4″ _module_preset=”default” hover_enabled=”0″ global_colors_info=”{}” open=”off” sticky_enabled=”0″]<\/p>\n Hikarazine\u2122 substrates contain fluor atoms, enhancing their solubility and bioavailability for small animal imaging. For optimal results, the final blood concentration should be around 13.6 \u00b5M, which corresponds to injecting 80 \u00b5L of Z103 or Z108 at 1.35 mM per mouse (assuming an average blood volume of 8 mL).<\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”Is Hikarazine\u2122 dependent on ATP?” _builder_version=”4.27.4″ _module_preset=”default” hover_enabled=”0″ global_colors_info=”{}” open=”off” sticky_enabled=”0″]<\/p>\n No, unlike firefly-based bioluminescent systems, marine-based bioluminescent systems, including Hikarazine\u2122, do not require ATP.<\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”Are Hikarazine\u2122 substrates patented?” _builder_version=”4.27.4″ _module_preset=”default” hover_enabled=”0″ global_colors_info=”{}” open=”off” sticky_enabled=”0″]<\/p>\n Yes, Hikarazine\u2122substrates are patented under EP3615516, US11938199, and JP7366752.<\/p>\n [\/et_pb_accordion_item][\/et_pb_accordion][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.16″ _module_preset=”default” background_color=”#D2C6D2″ background_enable_image=”off” background_size=”initial” global_colors_info=”{}”][et_pb_row column_structure=”1_2,1_2″ make_equal=”on” _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”1_2″ module_class=”centrer-verticalement” _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.16″ _module_preset=”default” text_font=”Rufina||||||||” text_text_color=”#4D1749″ text_font_size=”46px” text_line_height=”40px” custom_margin=”||0px||false|false” text_font_size_tablet=”37px” text_font_size_phone=”35px” text_font_size_last_edited=”on|phone” global_colors_info=”{}”]<\/p>\n We look [\/et_pb_text][\/et_pb_column][et_pb_column type=”1_2″ module_class=”centrer-verticalement” _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][dnxte_button button_text=”Contact us” button_link=”@ET-DC@eyJkeW5hbWljIjp0cnVlLCJjb250ZW50IjoicG9zdF9saW5rX3VybF9wYWdlIiwic2V0dGluZ3MiOnsicG9zdF9pZCI6IjI2NCJ9fQ==@” btn_show_hide=”off” dnxt_hover_2d=”dnxt-hover-float” dnxt_hover_bg_color=”#4D1749″ _builder_version=”4.27.4″ _dynamic_attributes=”button_link” _module_preset=”default” btn_fonts_font=”PT Serif||||||||” btn_fonts_text_color=”#FFFFFF” background_color=”#29102E” use_background_color_gradient=”on” background_color_gradient_type=”linear” background_color_gradient_direction=”-90deg” background_color_gradient_stops=”#29102e 0%|#4d1749 100%” background_color_gradient_start=”#29102e” background_color_gradient_end=”#4d1749″ custom_margin=”||||false|false” custom_padding=”20px|60px|20px|60px|true|true” border_width_all_btn_border=”0px” border_color_all_btn_border=”RGBA(255,255,255,0)” box_shadow_style=”preset3″ 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custom_margin=”0px||||false|false” global_colors_info=”{}”][\/et_pb_text][\/et_pb_column][et_pb_column type=”1_3″ _builder_version=”4.16″ _module_preset=”default” use_background_color_gradient=”on” background_color_gradient_direction=”45deg” background_color_gradient_stops=”#29102e 6%|#4d1749 65%” background_color_gradient_start=”#29102e” background_color_gradient_start_position=”6%” background_color_gradient_end=”#4d1749″ background_color_gradient_end_position=”65%” custom_padding=”78px|65px|78px|65px|true|true” global_colors_info=”{}”][et_pb_text _builder_version=”4.16″ _module_preset=”default” text_font=”Rufina||||||||” text_text_color=”#FFFFFF” text_font_size=”55px” text_line_height=”1em” text_font_size_tablet=”55px” text_font_size_phone=”40px” text_font_size_last_edited=”on|phone” global_colors_info=”{}”]<\/p>\n Even [\/et_pb_text][et_pb_divider show_divider=”off” _builder_version=”4.16″ _module_preset=”default” width=”100%” height=”50px” global_colors_info=”{}”][\/et_pb_divider][et_pb_text _builder_version=”4.16″ _module_preset=”default” text_font=”PT Serif||||||||” text_text_color=”#FFFFFF” text_font_size=”16px” background_layout=”dark” custom_margin=”0px||||false|false” global_colors_info=”{}”]<\/p>\n Cell-Free Systems Applications<\/p>\n [\/et_pb_text][\/et_pb_column][et_pb_column type=”1_3″ _builder_version=”4.16″ _module_preset=”default” background_image=”http:\/\/synthelis.com\/wp-content\/uploads\/2022\/01\/Synthelis-MemeUneProteineABesoindExprimerSonPotentiel-Part2.jpg” global_colors_info=”{}”][et_pb_text _builder_version=”4.16″ _module_preset=”default” text_font=”PT Serif||||||||” text_text_color=”#FFFFFF” text_font_size=”16px” background_layout=”dark” min_height=”50vh” min_height_tablet=”50vh” min_height_phone=”50vh” min_height_last_edited=”on|phone” custom_margin=”0px||||false|false” global_colors_info=”{}”][\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":" FAQ
forward to
your questions<\/p>\n
a protein
needs
to express
its potential.<\/p>\n