{"id":4615,"date":"2025-03-22T13:56:48","date_gmt":"2025-03-22T13:56:48","guid":{"rendered":"https:\/\/synthelis.com\/?page_id=4615"},"modified":"2025-07-10T14:06:15","modified_gmt":"2025-07-10T14:06:15","slug":"protein-expression-in-the-e-coli-system","status":"publish","type":"page","link":"https:\/\/synthelis.com\/en\/protein-expression-in-the-e-coli-system\/","title":{"rendered":"Protein expression in the E.coli system"},"content":{"rendered":"

[et_pb_section fb_built=”1″ _builder_version=”4.16″ _module_preset=”default” custom_padding=”0px||0px||true|false” global_colors_info=”{}”][et_pb_row column_structure=”3_5,2_5″ use_custom_gutter=”on” gutter_width=”1″ make_equal=”on” _builder_version=”4.27.4″ _module_preset=”default” width=”100%” max_width=”100%” min_height=”50vh” custom_padding=”0px||0px||false|false” global_colors_info=”{}”][et_pb_column type=”3_5″ _builder_version=”4.27.4″ _module_preset=”default” background_image=”http:\/\/synthelis.com\/wp-content\/uploads\/2025\/03\/Synthelis-Services-Expression-Proteines-Ecoli.webp” global_colors_info=”{}”][et_pb_text _builder_version=”4.16″ _module_preset=”default” text_font=”PT Serif||||||||” text_text_color=”#FFFFFF” text_font_size=”16px” background_layout=”dark” min_height=”33vh” custom_margin=”0px||||false|false” global_colors_info=”{}”][\/et_pb_text][\/et_pb_column][et_pb_column type=”2_5″ _builder_version=”4.16″ _module_preset=”default” use_background_color_gradient=”on” background_color_gradient_direction=”45deg” background_color_gradient_stops=”#29102e 6%|#4d1749 65%” background_color_gradient_start=”#29102e” background_color_gradient_start_position=”6%” background_color_gradient_end=”#4d1749″ background_color_gradient_end_position=”65%” custom_padding=”78px|65px|78px|65px|true|true” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” text_font=”Rufina||||||||” text_text_color=”#FFFFFF” text_font_size=”4em” text_line_height=”1em” header_font=”Rufina||||||||” header_text_color=”#FFFFFF” header_font_size=”55px” hover_enabled=”0″ header_font_size_tablet=”55px” header_font_size_phone=”40px” header_font_size_last_edited=”on|phone” global_colors_info=”{}” sticky_enabled=”0″]<\/p>\n

Protein expression in the E.coli system.<\/h1>\n

[\/et_pb_text][et_pb_divider show_divider=”off” _builder_version=”4.16″ _module_preset=”default” width=”100%” height=”50px” global_colors_info=”{}”][\/et_pb_divider][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” text_font=”Rufina||||||||” text_text_color=”#D2C6D2″ text_font_size=”40px” text_line_height=”1em” background_layout=”dark” custom_margin=”0px||||false|false” text_font_size_tablet=”40px” text_font_size_phone=”30px” text_font_size_last_edited=”on|phone” global_colors_info=”{}”]<\/p>\n

A classic system, mastered by experts<\/span><\/p>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ module_id=”introduction” _builder_version=”4.27.4″ _module_preset=”default” background_color=”#EDE8EC” global_colors_info=”{}”][et_pb_row column_structure=”1_4,1_4,1_4,1_4″ use_custom_gutter=”on” gutter_width=”2″ _builder_version=”4.27.4″ _module_preset=”default” max_width=”100%” custom_margin=”25px||25px||true|false” custom_padding=”||||false|false” global_colors_info=”{}”][et_pb_column type=”1_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][dnxte_blurb blurb_pre_switch=”on” blurb_pre_heading=” Step 1\/” pre_heading_tag=”p” blurb_heading=”Feasibility study ” dnxt_image=”http:\/\/synthelis.com\/wp-content\/uploads\/2022\/03\/Synthelis-Services-Etude-Faisabilite.jpg” dnxt_content_max_width=”90%” dnxt_pre_margin=”-30px||10px||false|false” dnxt_pre_padding=”20px|25px||25px|false|true” dnxt_header_padding=”|25px|30px|25px|false|true” dnxt_post_margin=”||||false|false” dnxt_post_padding=”||||false|false” dnxt_body_margin=”|||51px|false|false” dnxt_content_margin=”||||false|false” dnxt_content_padding=”25px|25px|25px|25px|false|true” heading_bg_show_hide=”on” heading_bg=”#FFFFFF” content_bg_show_hide=”on” content_bg=”#EDE8EC” btn_icon=”5||divi||400″ btn_icon_color=”#FFFFFF” _builder_version=”4.27.4″ _module_preset=”default” dnxt_pre_header_font=”|700|||||||” dnxt_heading_text_color=”#4D1749″ dnxt_heading_font_size=”25px” dnxt_body_font_size=”16px” dnxt_btn_text_font=”PT Serif||||||||” dnxt_btn_text_text_color=”#FFFFFF” background_color=”RGBA(255,255,255,0)” custom_margin=”||||false|false” custom_padding=”||||false|false” link_option_url=”https:\/\/synthelis.com\/?page_id=4597\/#faisabiliteecoli” border_width_all_dnxt_button_borders=”0px” box_shadow_style_dnxt_description_boxshadow=”preset3″ box_shadow_style_dnxt_button_boxshadow=”preset3″ global_colors_info=”{}”][\/dnxte_blurb][\/et_pb_column][et_pb_column type=”1_4″ _builder_version=”4.16″ 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dnxt_body_font_size=”16px” dnxt_btn_text_font=”PT Serif||||||||” dnxt_btn_text_text_color=”#FFFFFF” background_color=”RGBA(255,255,255,0)” custom_margin=”||||false|false” custom_padding=”||||false|false” link_option_url=”https:\/\/synthelis.com\/?page_id=4597\/#productionechantillonecoli” dnxt_heading_font_size_last_edited=”off|phone” border_width_all_dnxt_button_borders=”0px” box_shadow_style_dnxt_description_boxshadow=”preset3″ box_shadow_style_dnxt_button_boxshadow=”preset3″ global_colors_info=”{}”][\/dnxte_blurb][\/et_pb_column][et_pb_column type=”1_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][dnxte_blurb blurb_pre_switch=”on” blurb_pre_heading=” Step 3\/” pre_heading_tag=”p” blurb_heading=”Protein characterization” dnxt_image=”http:\/\/synthelis.com\/wp-content\/uploads\/2022\/03\/Synthelis-Partenaires.jpg” dnxt_content_max_width=”90%” dnxt_pre_margin=”-30px||10px||false|false” dnxt_pre_padding=”20px|25px||25px|false|true” dnxt_header_padding=”|25px|30px|25px|false|true” dnxt_post_margin=”||||false|false” dnxt_post_padding=”||||false|false” dnxt_body_margin=”|||51px|false|false” dnxt_content_margin=”||||false|false” dnxt_content_padding=”25px|25px|25px|25px|false|true” heading_bg_show_hide=”on” heading_bg=”#FFFFFF” content_bg_show_hide=”on” content_bg=”#EDE8EC” btn_icon=”5||divi||400″ btn_icon_color=”#FFFFFF” blurb_hover_switch=”on” dnxt_hover_effect=”dnxt-hover-hang” _builder_version=”4.27.4″ _module_preset=”default” dnxt_pre_header_font=”|700|||||||” dnxt_heading_text_color=”#4D1749″ dnxt_heading_font_size=”25px” dnxt_body_font_size=”16px” dnxt_btn_text_font=”PT Serif||||||||” dnxt_btn_text_text_color=”#FFFFFF” background_color=”RGBA(255,255,255,0)” custom_margin=”||||false|false” custom_padding=”||||false|false” link_option_url=”https:\/\/synthelis.com\/?page_id=4597\/#caracterisation” border_width_all_dnxt_button_borders=”0px” box_shadow_style_dnxt_description_boxshadow=”preset3″ box_shadow_style_dnxt_button_boxshadow=”preset3″ global_colors_info=”{}”][\/dnxte_blurb][\/et_pb_column][et_pb_column type=”1_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][dnxte_blurb blurb_pre_switch=”on” blurb_pre_heading=” Step 4\/” pre_heading_tag=”p” blurb_heading=”Production” dnxt_image=”http:\/\/synthelis.com\/wp-content\/uploads\/2025\/01\/Synthelis-Services-Production.jpg” dnxt_content_max_width=”90%” dnxt_pre_margin=”-30px||10px||false|false” dnxt_pre_padding=”20px|25px||25px|false|true” dnxt_header_padding=”|25px|30px|25px|false|true” dnxt_post_margin=”||||false|false” dnxt_post_padding=”||||false|false” dnxt_body_margin=”|||51px|false|false” dnxt_content_margin=”||||false|false” dnxt_content_padding=”25px|25px|25px|25px|false|true” heading_bg_show_hide=”on” heading_bg=”#FFFFFF” content_bg_show_hide=”on” content_bg=”#EDE8EC” btn_icon=”5||divi||400″ btn_icon_color=”#FFFFFF” blurb_hover_switch=”on” dnxt_hover_effect=”dnxt-hover-hang” 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_module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” text_font=”PT Serif||||||||” text_text_color=”#4D1749″ text_font_size=”1.9em” text_line_height=”1.3em” text_font_size_tablet=”1.7em” text_font_size_phone=”1.6em” text_font_size_last_edited=”on|phone” global_colors_info=”{}”]<\/p>\n

In addition to cell-free technologies, the Synthelis team also has extensive expertise in the production of proteins in the E. coli system. Depending on the type of protein, we can work with either cytoplasmic<\/strong> or periplasmic expression<\/strong>. Our services range from feasibility studies to production in 10 L fermenters.\u00a0<\/span><\/p>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ module_id=”faisabiliteecoli” _builder_version=”4.27.4″ _module_preset=”default” background_color=”#EDE8EC” global_colors_info=”{}”][et_pb_row _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” header_font=”PT Serif|700|||||||” header_text_color=”#4D1749″ header_2_font=”PT Serif|700|||||||” header_2_font_size=”30px” custom_margin=”||10px||false|false” header_2_font_size_tablet=”30px” header_2_font_size_phone=”30px” header_2_font_size_last_edited=”on|phone” border_color_bottom=”#EEEFF3″ global_colors_info=”{}”]<\/p>\n

Step 1\/<\/strong><\/span><\/p>\n

[\/et_pb_text][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” header_font=”PT Serif|700|||||||” header_text_color=”#4D1749″ header_2_font=”PT Serif|700|||||||” header_2_font_size=”30px” header_2_font_size_tablet=”30px” header_2_font_size_phone=”30px” header_2_font_size_last_edited=”on|phone” border_color_bottom=”#EEEFF3″ global_colors_info=”{}”]<\/p>\n

Feasibility study<\/h2>\n

[\/et_pb_text][et_pb_divider color=”#D2C6D2″ divider_position=”center” divider_weight=”3px” _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”0px||0px||true|false” global_colors_info=”{}”][\/et_pb_divider][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.16″ _module_preset=”default” custom_margin=”||||true|false” custom_padding=”||||false|false” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][dnxte_advanced_tab hover_effect=”dnxt-hover-fadeIn ” tab_hover_effect=”none” tab_wrapper_margin=”||||false|false” body_wrapper_margin=”|0px||0px|false|false” body_wrapper_padding=”|0px||0px|false|false” tab_container_bg_color=”RGBA(255,255,255,0)” tab_single_bg_color=”RGBA(255,255,255,0)” tab_active_bg_color=”RGBA(255,255,255,0)” content_container_bg_color=”RGBA(255,255,255,0)” _builder_version=”4.27.4″ _module_preset=”default” tab_title_font=”PT Serif|700|||||||” tab_title_text_color=”#D2C6D2″ tab_title_font_size=”25px” tab_active_title_text_color=”#4D1749″ width=”100%” max_width=”100%” custom_margin=”0px|0px||0px|false|false” custom_padding=”|0px||0px|false|false” border_width_bottom_tab_active=”3px” border_color_bottom_tab_active=”#4D1749″ global_colors_info=”{}”][dnxte_advanced_tab_item admin_label_text=”Discussion” tab_title=”Discussion” use_divi_library=”on” divi_library=”4381″ tab_item_margin=”||||false|false” _builder_version=”4.27.4″ _module_preset=”default” width=”1080px” max_width=”100%” global_colors_info=”{}”][\/dnxte_advanced_tab_item][dnxte_advanced_tab_item admin_label_text=”Study” tab_title=”Study” use_divi_library=”on” divi_library=”4387″ _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”][\/dnxte_advanced_tab_item][dnxte_advanced_tab_item admin_label_text=”Conception” tab_title=”Conception” use_divi_library=”on” divi_library=”4494″ _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”][\/dnxte_advanced_tab_item][dnxte_advanced_tab_item admin_label_text=”Screening” tab_title=”Screening” use_divi_library=”on” divi_library=”4498″ _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”][\/dnxte_advanced_tab_item][dnxte_advanced_tab_item admin_label_text=”Purification” tab_title=”Purification” use_divi_library=”on” divi_library=”4513″ tab_item_margin=”||||false|false” _builder_version=”4.27.4″ _module_preset=”default” width=”1080px” max_width=”100%” global_colors_info=”{}”][\/dnxte_advanced_tab_item][\/dnxte_advanced_tab][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ module_id=”productionechantillonecoli” _builder_version=”4.27.4″ _module_preset=”default” background_color=”#FFFFFF” global_colors_info=”{}”][et_pb_row _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” header_font=”PT Serif|700|||||||” header_text_color=”#4D1749″ header_2_font=”PT Serif|700|||||||” header_2_font_size=”30px” custom_margin=”||10px||false|false” header_2_font_size_tablet=”30px” header_2_font_size_phone=”30px” header_2_font_size_last_edited=”on|phone” border_color_bottom=”#EEEFF3″ global_colors_info=”{}”]<\/p>\n

Step 2\/<\/strong><\/span><\/p>\n

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Sample production<\/h2>\n

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When the best expression conditions are defined, a small-scale production is carried out to allow the development of the purification protocol and to estimate the production yield of the protein. The resulting purified product is then either shipped to the customer for testing or used for quality control testing at Synthelis.<\/span><\/p>\n

\u00a0<\/p>\n

[\/et_pb_text][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ module_id=”caracterisation” _builder_version=”4.27.4″ _module_preset=”default” background_color=”#D2C6D2″ global_colors_info=”{}”][et_pb_row _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” header_font=”PT Serif|700|||||||” header_text_color=”#4D1749″ header_2_font=”PT Serif|700|||||||” header_2_font_size=”30px” custom_margin=”||10px||false|false” header_2_font_size_tablet=”30px” header_2_font_size_phone=”30px” header_2_font_size_last_edited=”on|phone” border_color_bottom=”#EEEFF3″ global_colors_info=”{}”]<\/p>\n

Step 3\/<\/strong><\/span><\/p>\n

[\/et_pb_text][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” header_font=”PT Serif|700|||||||” header_text_color=”#4D1749″ header_2_font=”PT Serif|700|||||||” header_2_font_size=”30px” header_2_font_size_tablet=”30px” header_2_font_size_phone=”30px” header_2_font_size_last_edited=”on|phone” border_color_bottom=”#EEEFF3″ global_colors_info=”{}”]<\/p>\n

Protein Characterization<\/h2>\n

[\/et_pb_text][et_pb_divider color=”#72486E” divider_position=”center” divider_weight=”3px” _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”0px||0px||true|false” global_colors_info=”{}”][\/et_pb_divider][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.16″ _module_preset=”default” custom_margin=”||||true|false” custom_padding=”||||false|false” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][dnxte_advanced_tab hover_effect=”dnxt-hover-fadeIn ” tab_hover_effect=”none” tab_wrapper_margin=”||||false|false” body_wrapper_margin=”|0px||0px|false|false” body_wrapper_padding=”|0px||0px|false|false” tab_container_bg_color=”RGBA(255,255,255,0)” tab_single_bg_color=”RGBA(255,255,255,0)” tab_active_bg_color=”RGBA(255,255,255,0)” content_container_bg_color=”RGBA(255,255,255,0)” _builder_version=”4.27.4″ _module_preset=”default” tab_title_font=”PT Serif|700|||||||” tab_title_text_color=”#72486E” tab_title_font_size=”25px” tab_active_title_text_color=”#4D1749″ width=”100%” max_width=”100%” custom_margin=”0px|0px||0px|false|false” custom_padding=”|0px||0px|false|false” border_width_bottom_tab_active=”3px” border_color_bottom_tab_active=”#4D1749″ global_colors_info=”{}”][dnxte_advanced_tab_item admin_label_text=”Qualitative studies” tab_title=”Qualitative studies” use_divi_library=”on” divi_library=”4029″ tab_item_margin=”||||false|false” _builder_version=”4.27.4″ _module_preset=”default” width=”1080px” max_width=”100%” global_colors_info=”{}”][\/dnxte_advanced_tab_item][dnxte_advanced_tab_item admin_label_text=”Quantitative studies” tab_title=”Quantitative studies” use_divi_library=”on” divi_library=”4043″ _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”][\/dnxte_advanced_tab_item][dnxte_advanced_tab_item admin_label_text=”Interaction studies” tab_title=”Interaction studies” use_divi_library=”on” divi_library=”4044″ _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”][\/dnxte_advanced_tab_item][dnxte_advanced_tab_item admin_label_text=”Activity studies” tab_title=”Activity studies” use_divi_library=”on” divi_library=”4045″ _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”][\/dnxte_advanced_tab_item][dnxte_advanced_tab_item admin_label_text=”Structural studies” tab_title=”Structural studies” use_divi_library=”on” divi_library=”4046″ tab_item_margin=”||||false|false” _builder_version=”4.27.4″ _module_preset=”default” width=”1080px” max_width=”100%” global_colors_info=”{}”][\/dnxte_advanced_tab_item][\/dnxte_advanced_tab][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ module_id=”production” _builder_version=”4.27.4″ _module_preset=”default” background_color=”#FFFFFF” global_colors_info=”{}”][et_pb_row _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” header_font=”PT Serif|700|||||||” header_text_color=”#4D1749″ header_2_font=”PT Serif|700|||||||” header_2_font_size=”30px” custom_margin=”||10px||false|false” header_2_font_size_tablet=”30px” header_2_font_size_phone=”30px” header_2_font_size_last_edited=”on|phone” border_color_bottom=”#EEEFF3″ global_colors_info=”{}”]<\/p>\n

Step 4\/<\/strong><\/span><\/p>\n

[\/et_pb_text][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” header_font=”PT Serif|700|||||||” header_text_color=”#4D1749″ header_2_font=”PT Serif|700|||||||” header_2_font_size=”30px” header_2_font_size_tablet=”30px” header_2_font_size_phone=”30px” header_2_font_size_last_edited=”on|phone” border_color_bottom=”#EEEFF3″ global_colors_info=”{}”]<\/p>\n

Production<\/h2>\n

[\/et_pb_text][et_pb_divider color=”#D2C6D2″ divider_position=”center” divider_weight=”3px” _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”0px||0px||true|false” global_colors_info=”{}”][\/et_pb_divider][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_2,1_2″ _builder_version=”4.16″ _module_preset=”default” custom_margin=”||||true|false” custom_padding=”||||false|false” global_colors_info=”{}”][et_pb_column type=”1_2″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” text_font=”PT Serif||||||||” text_text_color=”#4D1749″ text_font_size=”1.4em” text_line_height=”1.4em” global_colors_info=”{}”]<\/p>\n

From design to purification, we optimize every step to maximize the expression and quality of your protein.
Depending on your needs, we can produce proteins on a small scale for screening or preliminary testing (volumes from 4 to 250 mL). Once the conditions have been optimized, production can be scaled up to several liters (1 L to 8 L, or even more) to obtain sufficient quantities, from milligrams to grams of purified protein.<\/span><\/p>\n

\u00a0<\/p>\n

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Process development and industrialization<\/strong><\/span><\/p>\n

Our team is always here to help you if you want to improve your production process or change scale. We have a 10 L fermenter, purification equipment and, above all, the expertise necessary to enable you to take the first key steps towards the development and ultimate success of your project. Please not hesitate to contact us if you require more information.<\/span><\/p>\n

\u00a0<\/p>\n

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Case studies<\/h2>\n

[\/et_pb_text][et_pb_divider color=”#D2C6D2″ divider_position=”center” divider_weight=”3px” _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”0px||0px||true|false” global_colors_info=”{}”][\/et_pb_divider][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_3,1_3,1_3″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”1_3″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” text_font=”PT Serif||||||||” text_text_color=”#4D1749″ text_font_size=”1.4em” text_line_height=”1.4em” global_colors_info=”{}”]<\/p>\n

Importation Hubspot<\/span><\/p>\n

<\/span><\/p>\n

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Importation Hubspot<\/span><\/p>\n

<\/span><\/p>\n

[\/et_pb_text][\/et_pb_column][et_pb_column type=”1_3″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” text_font=”PT Serif||||||||” text_text_color=”#4D1749″ text_font_size=”1.4em” text_line_height=”1.4em” global_colors_info=”{}”]<\/p>\n

Importation Hubspot<\/span><\/p>\n

<\/span><\/p>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.27.4″ _module_preset=”default” background_color=”#EDE8EC” background_enable_image=”off” background_size=”initial” custom_padding=”||80px||false|false” global_colors_info=”{}”][et_pb_row _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.16″ _module_preset=”default” text_font=”PT Serif|700|||||||” text_font_size=”30px” header_font=”PT Serif|700|||||||” header_text_color=”#4D1749″ header_3_font=”PT Serif|700|||||||” header_3_font_size=”30px” border_color_bottom=”#EEEFF3″ global_colors_info=”{}”]<\/p>\n

Find our services on:<\/p>\n

[\/et_pb_text][et_pb_divider color=”#D2C6D2″ divider_position=”center” _builder_version=”4.16″ _module_preset=”default” custom_margin=”0px||0px||true|false” global_colors_info=”{}”][\/et_pb_divider][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_3,1_3,1_3″ make_equal=”on” _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”1_3″ module_class=”centrer-verticalement” _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_image src=”http:\/\/synthelis.com\/wp-content\/uploads\/2022\/03\/Scientist.jpg” alt=”Scientist.com logo” title_text=”Scientist.com logo” url=”https:\/\/scientist.com\/” url_new_window=”on” _builder_version=”4.16″ _module_preset=”default” box_shadow_style=”preset3″ global_colors_info=”{}”][\/et_pb_image][\/et_pb_column][et_pb_column type=”1_3″ module_class=”centrer-verticalement” _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_image src=”http:\/\/synthelis.com\/wp-content\/uploads\/2022\/03\/ScienceExchange.jpg” alt=”Science Exchange Logo” title_text=”Science Exchange Logo” url=”https:\/\/ww2.scienceexchange.com\/s\/” url_new_window=”on” _builder_version=”4.16″ _module_preset=”default” box_shadow_style=”preset3″ global_colors_info=”{}”][\/et_pb_image][\/et_pb_column][et_pb_column type=”1_3″ module_class=”centrer-verticalement” _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_image src=”http:\/\/synthelis.com\/wp-content\/uploads\/2022\/03\/Labtoo.jpg” alt=”Labtoo Logo” title_text=”Labtoo Logo” url=”https:\/\/www.labtoo.com\/fr\/” url_new_window=”on” _builder_version=”4.16″ _module_preset=”default” box_shadow_style=”preset3″ global_colors_info=”{}”][\/et_pb_image][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.27.4″ _module_preset=”default” background_color=”RGBA(255,255,255,0)” background_enable_image=”off” background_size=”initial” positioning=”fixed” position_origin_f=”bottom_center” vertical_offset=”100px” z_index=”1000″ custom_padding=”0px||0px||true|false” global_colors_info=”{}”][et_pb_row make_equal=”on” _builder_version=”4.27.4″ _module_preset=”default” width=”90%” max_width=”100%” custom_margin=”0px||0px||true|false” global_colors_info=”{}”][et_pb_column type=”4_4″ module_class=”centrer-verticalement” _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][dnxte_button button_text=”Get a quote” button_link=”@ET-DC@eyJkeW5hbWljIjp0cnVlLCJjb250ZW50IjoicG9zdF9saW5rX3VybF9wYWdlIiwic2V0dGluZ3MiOnsicG9zdF9pZCI6IjEwNTMifX0=@” dnxt_button_alignment=”right” btn_show_hide=”off” dnxt_hover_2d=”dnxt-hover-float” dnxt_hover_bg_color=”#4D1749″ _builder_version=”4.27.4″ _dynamic_attributes=”button_link” _module_preset=”default” btn_fonts_font=”PT Serif||||||||” btn_fonts_text_color=”#FFFFFF” background_color=”#29102E” use_background_color_gradient=”on” background_color_gradient_type=”linear” background_color_gradient_direction=”-90deg” background_color_gradient_stops=”#29102e 0%|#4d1749 100%” background_color_gradient_start=”#29102e” background_color_gradient_end=”#4d1749″ custom_margin=”||||false|false” custom_padding=”20px|60px|20px|60px|true|true” border_width_all_btn_border=”0px” border_color_all_btn_border=”RGBA(255,255,255,0)” box_shadow_style=”preset3″ global_colors_info=”{}”][\/dnxte_button][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.16″ _module_preset=”default” background_color=”#D2C6D2″ background_enable_image=”off” background_size=”initial” global_colors_info=”{}”][et_pb_row column_structure=”1_2,1_2″ make_equal=”on” _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”1_2″ module_class=”centrer-verticalement” _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.16″ _module_preset=”default” text_font=”Rufina||||||||” text_text_color=”#4D1749″ text_font_size=”46px” text_line_height=”40px” custom_margin=”||0px||false|false” text_font_size_tablet=”37px” text_font_size_phone=”35px” text_font_size_last_edited=”on|phone” global_colors_info=”{}”]<\/p>\n

We look
forward to
your questions<\/p>\n

[\/et_pb_text][\/et_pb_column][et_pb_column type=”1_2″ module_class=”centrer-verticalement” _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][dnxte_button button_text=”Contact us” button_link=”@ET-DC@eyJkeW5hbWljIjp0cnVlLCJjb250ZW50IjoicG9zdF9saW5rX3VybF9wYWdlIiwic2V0dGluZ3MiOnsicG9zdF9pZCI6IjI2NCJ9fQ==@” btn_show_hide=”off” dnxt_hover_2d=”dnxt-hover-float” dnxt_hover_bg_color=”#4D1749″ _builder_version=”4.27.4″ _dynamic_attributes=”button_link” _module_preset=”default” btn_fonts_font=”PT Serif||||||||” btn_fonts_text_color=”#FFFFFF” background_color=”#29102E” use_background_color_gradient=”on” background_color_gradient_type=”linear” background_color_gradient_direction=”-90deg” background_color_gradient_stops=”#29102e 0%|#4d1749 100%” background_color_gradient_start=”#29102e” background_color_gradient_end=”#4d1749″ custom_margin=”||||false|false” custom_padding=”20px|60px|20px|60px|true|true” border_width_all_btn_border=”0px” border_color_all_btn_border=”RGBA(255,255,255,0)” box_shadow_style=”preset3″ global_colors_info=”{}”][\/dnxte_button][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.16″ _module_preset=”default” custom_padding=”0px||0px||true|false” global_colors_info=”{}”][et_pb_row column_structure=”1_3,1_3,1_3″ use_custom_gutter=”on” gutter_width=”1″ make_equal=”on” _builder_version=”4.16″ _module_preset=”default” width=”100%” max_width=”100%” min_height=”70vh” custom_padding=”0px||0px||false|false” global_colors_info=”{}”][et_pb_column type=”1_3″ _builder_version=”4.16″ _module_preset=”default” background_image=”http:\/\/synthelis.com\/wp-content\/uploads\/2022\/01\/Synthelis-MemeUneProteineABesoindExprimerSonPotentiel-Part1.jpg” global_colors_info=”{}”][et_pb_text _builder_version=”4.16″ _module_preset=”default” text_font=”PT Serif||||||||” text_text_color=”#FFFFFF” text_font_size=”16px” background_layout=”dark” min_height=”50vh” min_height_tablet=”50vh” min_height_phone=”50vh” min_height_last_edited=”on|phone” custom_margin=”0px||||false|false” global_colors_info=”{}”][\/et_pb_text][\/et_pb_column][et_pb_column type=”1_3″ _builder_version=”4.16″ _module_preset=”default” use_background_color_gradient=”on” background_color_gradient_direction=”45deg” background_color_gradient_stops=”#29102e 6%|#4d1749 65%” background_color_gradient_start=”#29102e” background_color_gradient_start_position=”6%” background_color_gradient_end=”#4d1749″ background_color_gradient_end_position=”65%” custom_padding=”78px|65px|78px|65px|true|true” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” text_font=”Rufina||||||||” text_text_color=”#FFFFFF” text_font_size=”55px” text_line_height=”1em” text_font_size_tablet=”55px” text_font_size_phone=”40px” text_font_size_last_edited=”on|phone” global_colors_info=”{}”]<\/p>\n

Even
a protein
needs
to express
its potential<\/p>\n

[\/et_pb_text][et_pb_divider show_divider=”off” _builder_version=”4.16″ _module_preset=”default” width=”100%” height=”50px” global_colors_info=”{}”][\/et_pb_divider][et_pb_text _builder_version=”4.16″ _module_preset=”default” text_font=”PT Serif||||||||” text_text_color=”#FFFFFF” text_font_size=”16px” background_layout=”dark” custom_margin=”0px||||false|false” global_colors_info=”{}”]<\/p>\n

Cell-Free Systems Applications<\/p>\n

[\/et_pb_text][\/et_pb_column][et_pb_column type=”1_3″ _builder_version=”4.16″ _module_preset=”default” background_image=”http:\/\/synthelis.com\/wp-content\/uploads\/2022\/01\/Synthelis-MemeUneProteineABesoindExprimerSonPotentiel-Part2.jpg” global_colors_info=”{}”][et_pb_text _builder_version=”4.16″ _module_preset=”default” text_font=”PT Serif||||||||” text_text_color=”#FFFFFF” text_font_size=”16px” background_layout=”dark” min_height=”50vh” min_height_tablet=”50vh” min_height_phone=”50vh” min_height_last_edited=”on|phone” custom_margin=”0px||||false|false” global_colors_info=”{}”][\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"

Protein expression in the E.coli system.A classic system, mastered by experts

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Step 1\/<\/p>\n\t\t\t\t\t\t\t\t

Feasibility study<\/h2>\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t<\/div>
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\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t<\/div><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","_surecart_dashboard_logo_width":"180px","_surecart_dashboard_show_logo":true,"_surecart_dashboard_navigation_orders":true,"_surecart_dashboard_navigation_invoices":true,"_surecart_dashboard_navigation_subscriptions":true,"_surecart_dashboard_navigation_downloads":true,"_surecart_dashboard_navigation_billing":true,"_surecart_dashboard_navigation_account":true,"footnotes":""},"folder":[51],"class_list":["post-4615","page","type-page","status-publish","hentry"],"yoast_head":"\nProtein expression in the E.coli system - Synthelis<\/title>\n<meta name=\"description\" content=\"Synthelis offers reliable E. coli protein expression services for efficient, high-quality production of recombinant proteins.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/synthelis.com\/en\/protein-expression-in-the-e-coli-system\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Protein expression in the E.coli system - 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