{"id":4563,"date":"2025-03-22T13:56:24","date_gmt":"2025-03-22T13:56:24","guid":{"rendered":"https:\/\/synthelis.com\/?page_id=4563"},"modified":"2025-07-11T16:24:24","modified_gmt":"2025-07-11T16:24:24","slug":"protein-expression-in-cell-free-system","status":"publish","type":"page","link":"https:\/\/synthelis.com\/en\/protein-expression-in-cell-free-system\/","title":{"rendered":"Protein expression in Cell-Free system"},"content":{"rendered":"

[et_pb_section fb_built=”1″ _builder_version=”4.16″ _module_preset=”default” custom_padding=”0px||0px||true|false” global_colors_info=”{}”][et_pb_row column_structure=”3_5,2_5″ use_custom_gutter=”on” gutter_width=”1″ make_equal=”on” _builder_version=”4.27.4″ _module_preset=”default” width=”100%” max_width=”100%” min_height=”50vh” custom_padding=”0px||0px||false|false” global_colors_info=”{}”][et_pb_column type=”3_5″ _builder_version=”4.27.4″ _module_preset=”default” background_image=”http:\/\/synthelis.com\/wp-content\/uploads\/2025\/01\/Synthelis-Services-Expression-Proteines-Cell-free-1.jpg” global_colors_info=”{}”][et_pb_text _builder_version=”4.16″ _module_preset=”default” text_font=”PT Serif||||||||” text_text_color=”#FFFFFF” text_font_size=”16px” background_layout=”dark” min_height=”33vh” custom_margin=”0px||||false|false” global_colors_info=”{}”][\/et_pb_text][\/et_pb_column][et_pb_column type=”2_5″ _builder_version=”4.16″ _module_preset=”default” use_background_color_gradient=”on” background_color_gradient_direction=”45deg” background_color_gradient_stops=”#29102e 6%|#4d1749 65%” background_color_gradient_start=”#29102e” background_color_gradient_start_position=”6%” background_color_gradient_end=”#4d1749″ background_color_gradient_end_position=”65%” custom_padding=”78px|65px|78px|65px|true|true” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” text_font=”Rufina||||||||” text_text_color=”#FFFFFF” text_font_size=”4em” text_line_height=”1em” header_font=”Rufina||||||||” header_text_color=”#FFFFFF” header_font_size=”55px” header_font_size_tablet=”55px” header_font_size_phone=”40px” header_font_size_last_edited=”on|phone” global_colors_info=”{}”]<\/p>\n

Cell-Free
Protein
Synthesis.<\/h1>\n

[\/et_pb_text][et_pb_divider show_divider=”off” _builder_version=”4.16″ _module_preset=”default” width=”100%” height=”50px” global_colors_info=”{}”][\/et_pb_divider][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” text_font=”Rufina||||||||” text_text_color=”#D2C6D2″ text_font_size=”40px” text_line_height=”1em” background_layout=”dark” custom_margin=”0px||||false|false” text_font_size_tablet=”40px” text_font_size_phone=”30px” text_font_size_last_edited=”on|phone” global_colors_info=”{}”]Proven expertise,
\nhigh-throughput platform:
\nfaster and smarter!<\/span>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ module_id=”introduction” _builder_version=”4.27.4″ _module_preset=”default” background_color=”#EDE8EC” global_colors_info=”{}”][et_pb_row column_structure=”1_4,1_4,1_4,1_4″ use_custom_gutter=”on” gutter_width=”2″ _builder_version=”4.27.4″ _module_preset=”default” max_width=”100%” custom_margin=”25px||25px||true|false” custom_padding=”||||false|false” global_colors_info=”{}”][et_pb_column type=”1_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][dnxte_blurb blurb_pre_switch=”on” blurb_pre_heading=” Step 1\/” pre_heading_tag=”p” blurb_heading=”Feasibility study ” dnxt_image=”http:\/\/synthelis.com\/wp-content\/uploads\/2022\/03\/Synthelis-Services-Etude-Faisabilite.jpg” dnxt_content_max_width=”90%” dnxt_pre_margin=”-30px||10px||false|false” dnxt_pre_padding=”20px|25px||25px|false|true” dnxt_header_padding=”|25px|30px|25px|false|true” dnxt_post_margin=”||||false|false” dnxt_post_padding=”||||false|false” dnxt_body_margin=”|||51px|false|false” dnxt_content_margin=”||||false|false” dnxt_content_padding=”25px|25px|25px|25px|false|true” heading_bg_show_hide=”on” heading_bg=”#FFFFFF” content_bg_show_hide=”on” content_bg=”#EDE8EC” btn_icon=”5||divi||400″ btn_icon_color=”#FFFFFF” _builder_version=”4.27.4″ _module_preset=”default” dnxt_pre_header_font=”|700|||||||” dnxt_heading_text_color=”#4D1749″ dnxt_heading_font_size=”25px” dnxt_body_font_size=”16px” dnxt_btn_text_font=”PT Serif||||||||” dnxt_btn_text_text_color=”#FFFFFF” background_color=”RGBA(255,255,255,0)” custom_margin=”||||false|false” custom_padding=”||||false|false” link_option_url=”https:\/\/synthelis.com\/en\/?page_id=4563\/#faisabilite” border_width_all_dnxt_button_borders=”0px” box_shadow_style_dnxt_description_boxshadow=”preset3″ box_shadow_style_dnxt_button_boxshadow=”preset3″ global_colors_info=”{}”][\/dnxte_blurb][\/et_pb_column][et_pb_column type=”1_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][dnxte_blurb blurb_pre_switch=”on” blurb_pre_heading=” \u00c9tape 2\/” pre_heading_tag=”p” blurb_heading=”Sample production” dnxt_image=”http:\/\/synthelis.com\/wp-content\/uploads\/2022\/03\/Synthelis-CaseStudies-Sonofi.jpg” dnxt_content_max_width=”90%” dnxt_pre_margin=”-30px||10px||false|false” dnxt_pre_padding=”20px|25px||25px|false|true” dnxt_header_padding=”|25px|30px|25px|false|true” dnxt_post_margin=”||||false|false” dnxt_post_padding=”||||false|false” dnxt_body_margin=”|||51px|false|false” dnxt_content_margin=”||||false|false” dnxt_content_padding=”25px|25px|25px|25px|false|true” heading_bg_show_hide=”on” heading_bg=”#FFFFFF” content_bg_show_hide=”on” content_bg=”#EDE8EC” btn_icon=”5||divi||400″ btn_icon_color=”#FFFFFF” blurb_hover_switch=”on” dnxt_hover_effect=”dnxt-hover-hang” _builder_version=”4.27.4″ _module_preset=”default” dnxt_pre_header_font=”|700|||||||” dnxt_heading_text_color=”#4D1749″ dnxt_heading_font_size=”25px” dnxt_body_font_size=”16px” dnxt_btn_text_font=”PT Serif||||||||” dnxt_btn_text_text_color=”#FFFFFF” background_color=”RGBA(255,255,255,0)” custom_margin=”||||false|false” custom_padding=”||||false|false” link_option_url=”https:\/\/synthelis.com\/en\/?page_id=4563\/#productionechantillon” dnxt_heading_font_size_last_edited=”off|phone” border_width_all_dnxt_button_borders=”0px” box_shadow_style_dnxt_description_boxshadow=”preset3″ box_shadow_style_dnxt_button_boxshadow=”preset3″ global_colors_info=”{}”][\/dnxte_blurb][\/et_pb_column][et_pb_column type=”1_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][dnxte_blurb blurb_pre_switch=”on” blurb_pre_heading=” Step 3\/” pre_heading_tag=”p” blurb_heading=”Protein characterization” dnxt_image=”http:\/\/synthelis.com\/wp-content\/uploads\/2022\/03\/Synthelis-Partenaires.jpg” dnxt_content_max_width=”90%” dnxt_pre_margin=”-30px||10px||false|false” dnxt_pre_padding=”20px|25px||25px|false|true” dnxt_header_padding=”|25px|30px|25px|false|true” dnxt_post_margin=”||||false|false” dnxt_post_padding=”||||false|false” dnxt_body_margin=”|||51px|false|false” dnxt_content_margin=”||||false|false” dnxt_content_padding=”25px|25px|25px|25px|false|true” heading_bg_show_hide=”on” heading_bg=”#FFFFFF” content_bg_show_hide=”on” content_bg=”#EDE8EC” btn_icon=”5||divi||400″ btn_icon_color=”#FFFFFF” blurb_hover_switch=”on” dnxt_hover_effect=”dnxt-hover-hang” _builder_version=”4.27.4″ _module_preset=”default” dnxt_pre_header_font=”|700|||||||” dnxt_heading_text_color=”#4D1749″ dnxt_heading_font_size=”25px” dnxt_body_font_size=”16px” dnxt_btn_text_font=”PT Serif||||||||” dnxt_btn_text_text_color=”#FFFFFF” background_color=”RGBA(255,255,255,0)” custom_margin=”||||false|false” custom_padding=”||||false|false” link_option_url=”https:\/\/synthelis.com\/en\/?page_id=4563\/#caracterisation” border_width_all_dnxt_button_borders=”0px” box_shadow_style_dnxt_description_boxshadow=”preset3″ box_shadow_style_dnxt_button_boxshadow=”preset3″ global_colors_info=”{}”][\/dnxte_blurb][\/et_pb_column][et_pb_column type=”1_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][dnxte_blurb blurb_pre_switch=”on” blurb_pre_heading=” \u00c9tape 4\/” pre_heading_tag=”p” blurb_heading=”Production” dnxt_image=”http:\/\/synthelis.com\/wp-content\/uploads\/2025\/01\/Synthelis-Services-Production.jpg” dnxt_content_max_width=”90%” dnxt_pre_margin=”-30px||10px||false|false” dnxt_pre_padding=”20px|25px||25px|false|true” dnxt_header_padding=”|25px|30px|25px|false|true” dnxt_post_margin=”||||false|false” dnxt_post_padding=”||||false|false” dnxt_body_margin=”|||51px|false|false” dnxt_content_margin=”||||false|false” dnxt_content_padding=”25px|25px|25px|25px|false|true” heading_bg_show_hide=”on” heading_bg=”#FFFFFF” content_bg_show_hide=”on” content_bg=”#EDE8EC” btn_icon=”5||divi||400″ btn_icon_color=”#FFFFFF” blurb_hover_switch=”on” dnxt_hover_effect=”dnxt-hover-hang” _builder_version=”4.27.4″ _module_preset=”default” dnxt_pre_header_font=”|700|||||||” dnxt_heading_text_color=”#4D1749″ dnxt_heading_font_size=”25px” dnxt_body_font_size=”16px” dnxt_btn_text_font=”PT Serif||||||||” dnxt_btn_text_text_color=”#FFFFFF” background_color=”RGBA(255,255,255,0)” custom_margin=”||||false|false” custom_padding=”||||false|false” link_option_url=”https:\/\/synthelis.com\/en\/?page_id=4563\/#production” border_width_all_dnxt_button_borders=”0px” box_shadow_style_dnxt_description_boxshadow=”preset3″ box_shadow_style_dnxt_button_boxshadow=”preset3″ global_colors_info=”{}”][\/dnxte_blurb][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.27.4″ _module_preset=”default” background_color=”#FFFFFF” global_colors_info=”{}”][et_pb_row column_structure=”1_2,1_2″ _builder_version=”4.16″ _module_preset=”default” custom_margin=”||||true|false” custom_padding=”||||false|false” global_colors_info=”{}”][et_pb_column type=”1_2″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” text_font=”PT Serif||||||||” text_text_color=”#4D1749″ text_font_size=”1.4em” text_line_height=”1.4em” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″]<\/p>\n

The following options for our cell-free expression offering are available:
– from our proprietary system based on E.coli lysate<\/span><\/strong>
For over 15 years we have developed and optimized an expression system based on an E. coli lysate offering high expression yields and delivering high-quality, well-conformed and active proteins. This quality is obtained by researching and defining the optimal expression conditions for the protein of interest.<\/span><\/p>\n

– From almost all existing eukaryotic systems:<\/span><\/strong> Rabbit Reticulocyte Lysate (RLL), Insect, CHO, Human\u2026<\/p>\n

– From a custom-developed lysate<\/span><\/strong> (cell type desired by the customer)<\/p>\n

 <\/p>\n

\n

Because we have mastered the expression of complex proteins and cell-free technology, we are able to achieve high yields for a wide range of functional proteins, including membrane proteins and cytotoxic proteins.
Overcomethe limits of cell-based systems!<\/p>\n<\/blockquote>\n

[\/et_pb_text][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” text_text_color=”#FFFFFF” text_font_size=”15px” background_color=”#4D1749″ background_layout=”dark” custom_padding=”10px||10px||true|false” global_colors_info=”{}”]<\/p>\n

The benefits of the Synthelis Cell-Free approach<\/strong><\/p>\n

[\/et_pb_text][dnxte_rating star_rating_alignment=”center” rating_color=”#4D1749″ star_size=”14px” _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”||||false|false” custom_padding=”||||false|false” global_colors_info=”{}”][\/dnxte_rating][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”-20px||0px||false|false” global_colors_info=”{}”]<\/p>\n

Preserved functionality <\/strong>with full size proteins.<\/strong><\/p>\n

[\/et_pb_text][et_pb_divider color=”#D2C6D2″ divider_position=”center” _builder_version=”4.16″ _module_preset=”default” custom_margin=”10px||10px||true|false” global_colors_info=”{}”][\/et_pb_divider][dnxte_rating star_rating=”3″ star_rating_alignment=”center” rating_color=”#4D1749″ star_size=”14px” _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”||||false|false” custom_padding=”||||false|false” global_colors_info=”{}”][\/dnxte_rating][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”-20px||0px||false|false” global_colors_info=”{}”]<\/p>\n

Quantity: <\/strong>from 10 \u00b5g to 100 mg<\/p>\n

[\/et_pb_text][et_pb_divider color=”#D2C6D2″ divider_position=”center” _builder_version=”4.16″ _module_preset=”default” custom_margin=”10px||10px||true|false” global_colors_info=”{}”][\/et_pb_divider][dnxte_rating star_rating_alignment=”center” rating_color=”#4D1749″ star_size=”14px” _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”||||false|false” custom_padding=”||||false|false” global_colors_info=”{}”][\/dnxte_rating][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”-20px||0px||false|false” global_colors_info=”{}”]<\/p>\n

Speed: <\/strong>feasibility studies are performed in 2-3 months and production of previously expressed proteins requires 1-2 weeks per mg of protein*.<\/p>\n

*Example: 1 week for 1 mg of CXCR4 or Bak protein. Lead times may vary depending on the results of the feasibility study or the expression level of each protein.<\/em><\/p>\n

[\/et_pb_text][et_pb_divider color=”#D2C6D2″ divider_position=”center” _builder_version=”4.16″ _module_preset=”default” custom_margin=”10px||10px||true|false” global_colors_info=”{}”][\/et_pb_divider][dnxte_rating star_rating=”4″ star_rating_alignment=”center” rating_color=”#4D1749″ star_size=”14px” _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”||||false|false” custom_padding=”||||false|false” global_colors_info=”{}”][\/dnxte_rating][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”-20px||0px||false|false” global_colors_info=”{}”]<\/p>\n

High success expression rate: <\/strong>over 90% of the proteins were successfully expressed.<\/p>\n

[\/et_pb_text][et_pb_divider color=”#D2C6D2″ divider_position=”center” _builder_version=”4.16″ _module_preset=”default” custom_margin=”10px||10px||true|false” global_colors_info=”{}”][\/et_pb_divider][dnxte_rating star_rating_alignment=”center” rating_color=”#4D1749″ star_size=”14px” _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”||||false|false” custom_padding=”||||false|false” global_colors_info=”{}”][\/dnxte_rating][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”-20px||0px||false|false” global_colors_info=”{}”]<\/p>\n

Functionality: <\/strong>80% of the proteins tested are functional.<\/p>\n

[\/et_pb_text][et_pb_divider color=”#4D1749″ divider_position=”center” divider_weight=”5px” _builder_version=”4.16″ _module_preset=”default” custom_margin=”10px||10px||true|false” global_colors_info=”{}”][\/et_pb_divider][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ module_id=”faisabilite” _builder_version=”4.27.4″ _module_preset=”default” background_color=”#EDE8EC” global_colors_info=”{}”][et_pb_row _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” header_font=”PT Serif|700|||||||” header_text_color=”#4D1749″ header_2_font=”PT Serif|700|||||||” header_2_font_size=”30px” custom_margin=”||10px||false|false” header_2_font_size_tablet=”30px” header_2_font_size_phone=”30px” header_2_font_size_last_edited=”on|phone” border_color_bottom=”#EEEFF3″ global_colors_info=”{}”]<\/p>\n

Step 1\/<\/strong><\/span><\/p>\n

[\/et_pb_text][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” header_font=”PT Serif|700|||||||” header_text_color=”#4D1749″ header_2_font=”PT Serif|700|||||||” header_2_font_size=”30px” header_2_font_size_tablet=”30px” header_2_font_size_phone=”30px” header_2_font_size_last_edited=”on|phone” border_color_bottom=”#EEEFF3″ global_colors_info=”{}”]<\/p>\n

Feasibility study<\/h2>\n

[\/et_pb_text][et_pb_divider color=”#D2C6D2″ divider_position=”center” divider_weight=”3px” _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”0px||0px||true|false” global_colors_info=”{}”][\/et_pb_divider][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.16″ _module_preset=”default” custom_margin=”||||true|false” custom_padding=”||||false|false” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][dnxte_advanced_tab hover_effect=”dnxt-hover-fadeIn ” tab_hover_effect=”none” tab_wrapper_margin=”||||false|false” body_wrapper_margin=”|0px||0px|false|false” body_wrapper_padding=”|0px||0px|false|false” tab_container_bg_color=”RGBA(255,255,255,0)” tab_single_bg_color=”RGBA(255,255,255,0)” tab_active_bg_color=”RGBA(255,255,255,0)” content_container_bg_color=”RGBA(255,255,255,0)” _builder_version=”4.27.4″ _module_preset=”default” tab_title_font=”PT Serif|700|||||||” tab_title_text_color=”#D2C6D2″ tab_title_font_size=”25px” tab_active_title_text_color=”#4D1749″ width=”100%” max_width=”100%” custom_margin=”0px|0px||0px|false|false” custom_padding=”|0px||0px|false|false” border_width_bottom_tab_active=”3px” border_color_bottom_tab_active=”#4D1749″ global_colors_info=”{}”][dnxte_advanced_tab_item admin_label_text=”Discussion” tab_title=”Discussion” use_divi_library=”on” divi_library=”4381″ tab_item_margin=”||||false|false” _builder_version=”4.27.4″ _module_preset=”default” width=”1080px” max_width=”100%” global_colors_info=”{}”][\/dnxte_advanced_tab_item][dnxte_advanced_tab_item admin_label_text=”Study” tab_title=”Study” use_divi_library=”on” divi_library=”4387″ _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”][\/dnxte_advanced_tab_item][dnxte_advanced_tab_item admin_label_text=”Conception” tab_title=”Conception” use_divi_library=”on” divi_library=”4494″ _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”][\/dnxte_advanced_tab_item][dnxte_advanced_tab_item admin_label_text=”Screening” tab_title=”Screening” use_divi_library=”on” divi_library=”4498″ _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”][\/dnxte_advanced_tab_item][dnxte_advanced_tab_item admin_label_text=”Purification” tab_title=”Purification” use_divi_library=”on” divi_library=”4513″ tab_item_margin=”||||false|false” _builder_version=”4.27.4″ _module_preset=”default” width=”1080px” max_width=”100%” global_colors_info=”{}”][\/dnxte_advanced_tab_item][\/dnxte_advanced_tab][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ module_id=”productionechantillon” _builder_version=”4.27.4″ _module_preset=”default” background_color=”#FFFFFF” global_colors_info=”{}”][et_pb_row _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” header_font=”PT Serif|700|||||||” header_text_color=”#4D1749″ header_2_font=”PT Serif|700|||||||” header_2_font_size=”30px” custom_margin=”||10px||false|false” header_2_font_size_tablet=”30px” header_2_font_size_phone=”30px” header_2_font_size_last_edited=”on|phone” border_color_bottom=”#EEEFF3″ global_colors_info=”{}”]<\/p>\n

Step 2\/<\/strong><\/span><\/p>\n

[\/et_pb_text][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” header_font=”PT Serif|700|||||||” header_text_color=”#4D1749″ header_2_font=”PT Serif|700|||||||” header_2_font_size=”30px” header_2_font_size_tablet=”30px” header_2_font_size_phone=”30px” header_2_font_size_last_edited=”on|phone” border_color_bottom=”#EEEFF3″ global_colors_info=”{}”]<\/p>\n

Sample production<\/h2>\n

[\/et_pb_text][et_pb_divider color=”#D2C6D2″ divider_position=”center” divider_weight=”3px” _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”0px||0px||true|false” global_colors_info=”{}”][\/et_pb_divider][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_2,1_2″ _builder_version=”4.16″ _module_preset=”default” custom_margin=”||||true|false” custom_padding=”||||false|false” global_colors_info=”{}”][et_pb_column type=”1_2″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” text_font=”PT Serif||||||||” text_text_color=”#4D1749″ text_font_size=”1.4em” text_line_height=”1.4em” global_colors_info=”{}”]<\/p>\n

When the best expression conditions are defined, a small-scale production is carried out to allow the development of the purification protocol and to estimate the production yield of the protein. The resulting purified product is then either shipped to the customer for testing or used for quality control testing at Synthelis.<\/span><\/p>\n

[\/et_pb_text][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ module_id=”caracterisation” _builder_version=”4.27.4″ _module_preset=”default” background_color=”#D2C6D2″ global_colors_info=”{}”][et_pb_row _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” header_font=”PT Serif|700|||||||” header_text_color=”#4D1749″ header_2_font=”PT Serif|700|||||||” header_2_font_size=”30px” custom_margin=”||10px||false|false” header_2_font_size_tablet=”30px” header_2_font_size_phone=”30px” header_2_font_size_last_edited=”on|phone” border_color_bottom=”#EEEFF3″ global_colors_info=”{}”]<\/p>\n

Step 3\/<\/strong><\/span><\/p>\n

[\/et_pb_text][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” header_font=”PT Serif|700|||||||” header_text_color=”#4D1749″ header_2_font=”PT Serif|700|||||||” header_2_font_size=”30px” header_2_font_size_tablet=”30px” header_2_font_size_phone=”30px” header_2_font_size_last_edited=”on|phone” border_color_bottom=”#EEEFF3″ global_colors_info=”{}”]<\/p>\n

Protein Characterization<\/h2>\n

[\/et_pb_text][et_pb_divider color=”#72486E” divider_position=”center” divider_weight=”3px” _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”0px||0px||true|false” global_colors_info=”{}”][\/et_pb_divider][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.16″ _module_preset=”default” custom_margin=”||||true|false” custom_padding=”||||false|false” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][dnxte_advanced_tab hover_effect=”dnxt-hover-fadeIn ” tab_hover_effect=”none” tab_wrapper_margin=”||||false|false” body_wrapper_margin=”|0px||0px|false|false” body_wrapper_padding=”|0px||0px|false|false” tab_container_bg_color=”RGBA(255,255,255,0)” tab_single_bg_color=”RGBA(255,255,255,0)” tab_active_bg_color=”RGBA(255,255,255,0)” content_container_bg_color=”RGBA(255,255,255,0)” _builder_version=”4.27.4″ _module_preset=”default” tab_title_font=”PT Serif|700|||||||” tab_title_text_color=”#72486E” tab_title_font_size=”25px” tab_active_title_text_color=”#4D1749″ width=”100%” max_width=”100%” custom_margin=”0px|0px||0px|false|false” custom_padding=”|0px||0px|false|false” border_width_bottom_tab_active=”3px” border_color_bottom_tab_active=”#4D1749″ global_colors_info=”{}”][dnxte_advanced_tab_item admin_label_text=”\u00c9tudes qualitatives” tab_title=”Qualitative studies” use_divi_library=”on” divi_library=”4029″ tab_item_margin=”||||false|false” _builder_version=”4.27.4″ _module_preset=”default” width=”1080px” max_width=”100%” global_colors_info=”{}”][\/dnxte_advanced_tab_item][dnxte_advanced_tab_item admin_label_text=”\u00c9tude quantitatives” tab_title=”Quantitative studies” use_divi_library=”on” divi_library=”4043″ _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”][\/dnxte_advanced_tab_item][dnxte_advanced_tab_item admin_label_text=”\u00c9tudes des interactions” tab_title=”Interaction studies” use_divi_library=”on” divi_library=”4044″ _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”][\/dnxte_advanced_tab_item][dnxte_advanced_tab_item admin_label_text=”\u00c9tude d’activit\u00e9” tab_title=”Activity studies” use_divi_library=”on” divi_library=”4045″ _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”][\/dnxte_advanced_tab_item][dnxte_advanced_tab_item admin_label_text=”\u00c9tudes structurales” tab_title=”Structural studies” use_divi_library=”on” divi_library=”4046″ tab_item_margin=”||||false|false” _builder_version=”4.27.4″ _module_preset=”default” width=”1080px” max_width=”100%” global_colors_info=”{}”][\/dnxte_advanced_tab_item][\/dnxte_advanced_tab][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ module_id=”production” _builder_version=”4.27.4″ _module_preset=”default” background_color=”#EDE8EC” global_colors_info=”{}”][et_pb_row _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” header_font=”PT Serif|700|||||||” header_text_color=”#4D1749″ header_2_font=”PT Serif|700|||||||” header_2_font_size=”30px” custom_margin=”||10px||false|false” header_2_font_size_tablet=”30px” header_2_font_size_phone=”30px” header_2_font_size_last_edited=”on|phone” border_color_bottom=”#EEEFF3″ global_colors_info=”{}”]<\/p>\n

Step 4\/<\/strong><\/span><\/p>\n

[\/et_pb_text][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” header_font=”PT Serif|700|||||||” header_text_color=”#4D1749″ header_2_font=”PT Serif|700|||||||” header_2_font_size=”30px” header_2_font_size_tablet=”30px” header_2_font_size_phone=”30px” header_2_font_size_last_edited=”on|phone” border_color_bottom=”#EEEFF3″ global_colors_info=”{}”]<\/p>\n

Production<\/h2>\n

[\/et_pb_text][et_pb_divider color=”#D2C6D2″ divider_position=”center” divider_weight=”3px” _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”0px||0px||true|false” global_colors_info=”{}”][\/et_pb_divider][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_2,1_2″ _builder_version=”4.16″ _module_preset=”default” custom_margin=”||||true|false” custom_padding=”||||false|false” global_colors_info=”{}”][et_pb_column type=”1_2″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” text_font=”PT Serif||||||||” text_text_color=”#4D1749″ text_font_size=”1.4em” text_line_height=”1.4em” global_colors_info=”{}”]<\/p>\n

From design to purification, we optimize every step to maximize the expression and quality of your protein. Depending on your needs, cell-free production can be adapted to different scales. For screening or optimization phases, we produce small volumes (from a few microliters to a few milliliters), enabling rapid, parallel expression of several variants of a protein. This approach is ideal for testing different expression conditions, optimizing a protein’s structure or selecting the best functional version before scaling up.<\/span><\/p>\n

[\/et_pb_text][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” text_font=”PT Serif||||||||” text_text_color=”#4D1749″ text_font_size=”1.4em” text_line_height=”1.4em” global_colors_info=”{}”]<\/p>\n

When larger quantities are required, production can be rapidly scaled up to several milliliters or liters, using the optimized conditions defined during the development phase. Thanks to our mastery of cell-free systems, we can ensure efficient expression with high yields, suitable for applications ranging from basic research to protein production for preclinical testing or industrial bioprocessing.
\nThe flexibility of cell-free also means that production can be rapidly adjusted to meet requirements, without the delays and constraints associated with cell culture. Whether you need to produce a few micrograms of rare proteins or several milligrams for functional or structural studies, our expertise guarantees optimized, reproducible production.<\/span><\/p>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ module_id=”introduction” _builder_version=”4.27.4″ _module_preset=”default” background_color=”#FFFFFF” global_colors_info=”{}”][et_pb_row _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.4″ _module_preset=”default” header_font=”PT Serif|700|||||||” header_text_color=”#4D1749″ header_2_font=”PT Serif|700|||||||” header_2_font_size=”30px” header_2_font_size_tablet=”30px” header_2_font_size_phone=”30px” header_2_font_size_last_edited=”on|phone” border_color_bottom=”#EEEFF3″ global_colors_info=”{}”]<\/p>\n

Case studies<\/h2>\n

[\/et_pb_text][et_pb_divider color=”#D2C6D2″ divider_position=”center” divider_weight=”3px” _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”0px||0px||true|false” global_colors_info=”{}”][\/et_pb_divider][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_2,1_2″ make_equal=”on” _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”1_2″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][dnxte_blurb blurb_pre_switch=”on” blurb_pre_heading=”Research project designed for Sanofi Pasteur (pdf)” pre_heading_tag=”p” blurb_heading=”Solubilisation of a strongly aggregated vaccine antigen” heading_tag=”h4″ dnxt_image=”http:\/\/synthelis.com\/wp-content\/uploads\/2022\/03\/CaseStudy-SanofiPasteur-Vignette-2.jpg” dnxt_content_max_width=”90%” dnxt_pre_margin=”-30px||10px||false|false” dnxt_pre_padding=”20px|25px||25px|false|true” dnxt_header_padding=”|25px|30px|25px|false|true” dnxt_post_margin=”||||false|false” dnxt_post_padding=”||||false|false” dnxt_body_margin=”|||51px|false|false” dnxt_content_margin=”||||false|false” heading_bg_show_hide=”on” heading_bg=”#FFFFFF” btn_icon=”5||divi||400″ btn_icon_color=”#FFFFFF” blurb_hover_switch=”on” dnxt_hover_effect=”dnxt-hover-wobble-to-bottom-right” _builder_version=”4.27.4″ _module_preset=”default” dnxt_pre_header_font=”|700|||||||” dnxt_heading_text_color=”#4D1749″ dnxt_heading_font_size=”30px” dnxt_btn_text_font=”PT Serif||||||||” dnxt_btn_text_text_color=”#FFFFFF” background_color=”RGBA(255,255,255,0)” custom_margin=”||||false|false” custom_padding=”||||false|false” link_option_url=”https:\/\/hello.synthelis.com\/en\/etude-de-cas-synthelis-sanofi-0″ link_option_url_new_window=”on” border_width_all_dnxt_button_borders=”0px” box_shadow_style_dnxt_description_boxshadow=”preset3″ box_shadow_style_dnxt_button_boxshadow=”preset3″ global_colors_info=”{}”][\/dnxte_blurb][\/et_pb_column][et_pb_column type=”1_2″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.27.4″ _module_preset=”default” background_color=”#EDE8EC” background_enable_image=”off” background_size=”initial” custom_padding=”||80px||false|false” global_colors_info=”{}”][et_pb_row _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.16″ _module_preset=”default” text_font=”PT Serif|700|||||||” text_font_size=”30px” header_font=”PT Serif|700|||||||” header_text_color=”#4D1749″ header_3_font=”PT Serif|700|||||||” header_3_font_size=”30px” border_color_bottom=”#EEEFF3″ global_colors_info=”{}”]<\/p>\n

Find our services on:<\/p>\n

[\/et_pb_text][et_pb_divider color=”#D2C6D2″ divider_position=”center” _builder_version=”4.16″ _module_preset=”default” custom_margin=”0px||0px||true|false” global_colors_info=”{}”][\/et_pb_divider][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_3,1_3,1_3″ make_equal=”on” _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”1_3″ module_class=”centrer-verticalement” _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_image src=”http:\/\/synthelis.com\/wp-content\/uploads\/2022\/03\/Scientist.jpg” alt=”Scientist.com logo” title_text=”Scientist.com logo” url=”https:\/\/scientist.com\/” url_new_window=”on” _builder_version=”4.16″ _module_preset=”default” box_shadow_style=”preset3″ global_colors_info=”{}”][\/et_pb_image][\/et_pb_column][et_pb_column type=”1_3″ module_class=”centrer-verticalement” _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_image src=”http:\/\/synthelis.com\/wp-content\/uploads\/2022\/03\/ScienceExchange.jpg” alt=”Science Exchange Logo” title_text=”Science Exchange Logo” url=”https:\/\/ww2.scienceexchange.com\/s\/” url_new_window=”on” _builder_version=”4.16″ _module_preset=”default” box_shadow_style=”preset3″ global_colors_info=”{}”][\/et_pb_image][\/et_pb_column][et_pb_column type=”1_3″ module_class=”centrer-verticalement” _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_image src=”http:\/\/synthelis.com\/wp-content\/uploads\/2022\/03\/Labtoo.jpg” alt=”Labtoo Logo” title_text=”Labtoo Logo” url=”https:\/\/www.labtoo.com\/fr\/” url_new_window=”on” _builder_version=”4.16″ _module_preset=”default” box_shadow_style=”preset3″ global_colors_info=”{}”][\/et_pb_image][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.27.4″ _module_preset=”default” background_color=”RGBA(255,255,255,0)” background_enable_image=”off” background_size=”initial” positioning=”fixed” position_origin_f=”bottom_center” vertical_offset=”100px” z_index=”1000″ custom_padding=”0px||0px||true|false” global_colors_info=”{}”][et_pb_row make_equal=”on” _builder_version=”4.27.4″ _module_preset=”default” width=”90%” max_width=”100%” custom_margin=”0px||0px||true|false” global_colors_info=”{}”][et_pb_column type=”4_4″ module_class=”centrer-verticalement” _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][dnxte_button button_text=”Get a quote” button_link=”@ET-DC@eyJkeW5hbWljIjp0cnVlLCJjb250ZW50IjoicG9zdF9saW5rX3VybF9wYWdlIiwic2V0dGluZ3MiOnsicG9zdF9pZCI6IjEwNTMifX0=@” dnxt_button_alignment=”right” btn_show_hide=”off” dnxt_hover_2d=”dnxt-hover-float” dnxt_hover_bg_color=”#4D1749″ _builder_version=”4.27.4″ _dynamic_attributes=”button_link” _module_preset=”default” btn_fonts_font=”PT Serif||||||||” btn_fonts_text_color=”#FFFFFF” background_color=”#29102E” use_background_color_gradient=”on” background_color_gradient_type=”linear” background_color_gradient_direction=”-90deg” background_color_gradient_stops=”#29102e 0%|#4d1749 100%” background_color_gradient_start=”#29102e” background_color_gradient_end=”#4d1749″ custom_margin=”||||false|false” custom_padding=”20px|60px|20px|60px|true|true” border_width_all_btn_border=”0px” border_color_all_btn_border=”RGBA(255,255,255,0)” box_shadow_style=”preset3″ global_colors_info=”{}”][\/dnxte_button][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.16″ _module_preset=”default” background_color=”#D2C6D2″ background_enable_image=”off” background_size=”initial” global_colors_info=”{}”][et_pb_row column_structure=”1_2,1_2″ make_equal=”on” _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”1_2″ module_class=”centrer-verticalement” _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.16″ _module_preset=”default” text_font=”Rufina||||||||” text_text_color=”#4D1749″ text_font_size=”46px” text_line_height=”40px” custom_margin=”||0px||false|false” text_font_size_tablet=”37px” text_font_size_phone=”35px” text_font_size_last_edited=”on|phone” global_colors_info=”{}”]<\/p>\n

We look
forward to
your questions<\/p>\n

[\/et_pb_text][\/et_pb_column][et_pb_column type=”1_2″ module_class=”centrer-verticalement” _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][dnxte_button button_text=”Contact us” button_link=”@ET-DC@eyJkeW5hbWljIjp0cnVlLCJjb250ZW50IjoicG9zdF9saW5rX3VybF9wYWdlIiwic2V0dGluZ3MiOnsicG9zdF9pZCI6IjI2NCJ9fQ==@” btn_show_hide=”off” dnxt_hover_2d=”dnxt-hover-float” dnxt_hover_bg_color=”#4D1749″ _builder_version=”4.27.4″ _dynamic_attributes=”button_link” _module_preset=”default” btn_fonts_font=”PT Serif||||||||” btn_fonts_text_color=”#FFFFFF” background_color=”#29102E” use_background_color_gradient=”on” background_color_gradient_type=”linear” background_color_gradient_direction=”-90deg” background_color_gradient_stops=”#29102e 0%|#4d1749 100%” background_color_gradient_start=”#29102e” background_color_gradient_end=”#4d1749″ custom_margin=”||||false|false” custom_padding=”20px|60px|20px|60px|true|true” border_width_all_btn_border=”0px” border_color_all_btn_border=”RGBA(255,255,255,0)” box_shadow_style=”preset3″ global_colors_info=”{}”][\/dnxte_button][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.16″ _module_preset=”default” custom_padding=”0px||0px||true|false” global_colors_info=”{}”][et_pb_row column_structure=”1_3,1_3,1_3″ use_custom_gutter=”on” gutter_width=”1″ make_equal=”on” _builder_version=”4.16″ _module_preset=”default” width=”100%” max_width=”100%” min_height=”70vh” custom_padding=”0px||0px||false|false” global_colors_info=”{}”][et_pb_column type=”1_3″ _builder_version=”4.16″ _module_preset=”default” background_image=”http:\/\/synthelis.com\/wp-content\/uploads\/2022\/01\/Synthelis-MemeUneProteineABesoindExprimerSonPotentiel-Part1.jpg” global_colors_info=”{}”][et_pb_text _builder_version=”4.16″ _module_preset=”default” text_font=”PT Serif||||||||” text_text_color=”#FFFFFF” text_font_size=”16px” background_layout=”dark” min_height=”50vh” min_height_tablet=”50vh” min_height_phone=”50vh” min_height_last_edited=”on|phone” custom_margin=”0px||||false|false” global_colors_info=”{}”][\/et_pb_text][\/et_pb_column][et_pb_column type=”1_3″ _builder_version=”4.16″ _module_preset=”default” use_background_color_gradient=”on” background_color_gradient_direction=”45deg” background_color_gradient_stops=”#29102e 6%|#4d1749 65%” background_color_gradient_start=”#29102e” background_color_gradient_start_position=”6%” background_color_gradient_end=”#4d1749″ background_color_gradient_end_position=”65%” custom_padding=”78px|65px|78px|65px|true|true” global_colors_info=”{}”][et_pb_text _builder_version=”4.16″ _module_preset=”default” text_font=”Rufina||||||||” text_text_color=”#FFFFFF” text_font_size=”55px” text_line_height=”1em” text_font_size_tablet=”55px” text_font_size_phone=”40px” text_font_size_last_edited=”on|phone” global_colors_info=”{}”]<\/p>\n

Even
a protein
needs
to express
its potential.<\/p>\n

[\/et_pb_text][et_pb_divider show_divider=”off” _builder_version=”4.16″ _module_preset=”default” width=”100%” height=”50px” global_colors_info=”{}”][\/et_pb_divider][et_pb_text _builder_version=”4.16″ _module_preset=”default” text_font=”PT Serif||||||||” text_text_color=”#FFFFFF” text_font_size=”16px” background_layout=”dark” custom_margin=”0px||||false|false” global_colors_info=”{}”]<\/p>\n

Cell-Free Systems Applications<\/p>\n

[\/et_pb_text][\/et_pb_column][et_pb_column type=”1_3″ _builder_version=”4.16″ _module_preset=”default” background_image=”http:\/\/synthelis.com\/wp-content\/uploads\/2022\/01\/Synthelis-MemeUneProteineABesoindExprimerSonPotentiel-Part2.jpg” global_colors_info=”{}”][et_pb_text _builder_version=”4.16″ _module_preset=”default” text_font=”PT Serif||||||||” text_text_color=”#FFFFFF” text_font_size=”16px” background_layout=”dark” min_height=”50vh” min_height_tablet=”50vh” min_height_phone=”50vh” min_height_last_edited=”on|phone” custom_margin=”0px||||false|false” global_colors_info=”{}”][\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"

Cell-FreeProteinSynthesis.Proven expertise, high-throughput platform: faster and smarter!

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Step 1\/<\/p>\n\t\t\t\t\t\t\t\t

Feasibility study<\/h2>\n\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t\t\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t<\/div>
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