[et_pb_section fb_built=”1″ _builder_version=”4.16″ _module_preset=”default” use_background_color_gradient=”on” background_color_gradient_direction=”45deg” background_color_gradient_stops=”#29102e 6%|#4d1749 65%” background_color_gradient_start=”#29102e” background_color_gradient_start_position=”6%” background_color_gradient_end=”#4d1749″ background_color_gradient_end_position=”65%” custom_padding=”0px||0px||true|false” global_colors_info=”{}”][et_pb_row use_custom_gutter=”on” gutter_width=”1″ make_equal=”on” _builder_version=”4.16″ _module_preset=”default” custom_padding=”0px||0px||false|false” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” background_color_gradient_direction=”45deg” background_color_gradient_start=”#29102e” background_color_gradient_end=”#4d1749″ custom_padding=”78px||78px||true|true” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.8″ _module_preset=”default” text_font=”Rufina||||||||” text_text_color=”#FFFFFF” text_font_size=”4em” text_line_height=”1em” header_font=”Rufina||||||||” header_text_color=”#FFFFFF” header_font_size=”55px” header_font_size_tablet=”55px” header_font_size_phone=”40px” header_font_size_last_edited=”on|phone” global_colors_info=”{}”]<\/p>\n
[\/et_pb_text][et_pb_divider show_divider=”off” _builder_version=”4.16″ _module_preset=”default” width=”100%” height=”50px” global_colors_info=”{}”][\/et_pb_divider][et_pb_divider show_divider=”off” _builder_version=”4.16″ _module_preset=”default” width=”100%” height=”50px” global_colors_info=”{}”][\/et_pb_divider][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ module_id=”faqbio” _builder_version=”4.27.8″ _module_preset=”default” background_color=”#FFFFFF” custom_padding=”||80px||false|false” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″][et_pb_row _builder_version=”4.27.8″ _module_preset=”default” custom_margin=”||||true|false” custom_padding=”30px||||false|false” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.8″ _module_preset=”default” text_font_size=”1.9em” text_line_height=”1.3em” text_text_color=”#4D1749″ text_font=”–et_global_body_font||||||||” hover_enabled=”0″ sticky_enabled=”0″]<\/p>\n
Find answers to frequently asked questions about bioluminescence, luciferases and bioluminescent substrates, including their use in assays and imaging for life science research.<\/p>\n
[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ module_id=”faqbio” _builder_version=”4.27.4″ _module_preset=”default” background_color=”#EDE8EC” custom_padding=”||80px||false|false” global_colors_info=”{}”][et_pb_row _builder_version=”4.27.8″ _module_preset=”default” custom_margin=”||||true|false” custom_padding=”30px||||false|false” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_accordion open_toggle_text_color=”#4D1749″ open_toggle_background_color=”RGBA(255,255,255,0)” closed_toggle_background_color=”RGBA(255,255,255,0)” toggle_icon=”||divi||400″ icon_color=”#4D1749″ use_icon_font_size=”on” icon_font_size=”24px” _builder_version=”4.27.8″ _module_preset=”default” toggle_text_color=”#4D1749″ toggle_font=”PT Serif|700|||||||” toggle_font_size=”24px” body_font=”PT Serif||||||||” body_font_size=”1.4em” body_line_height=”1.4em” background_color=”RGBA(255,255,255,0)” border_width_all=”0px” border_style_all=”solid” border_width_bottom=”1px” border_color_bottom=”#4D1749″ global_colors_info=”{}” toggle_icon__hover_enabled=”off|desktop” toggle_icon__hover=”\ue044||divi||400″][et_pb_accordion_item title=”Which luciferases are compatible with Hikarazine\u2122?” open=”on” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}”]<\/p>\n
Hikarazine\u2122 has been specifically designed for use with luciferase enzymes derived from Oplophorus gracilirostris<\/i>.<\/p>\n
[\/et_pb_accordion_item][et_pb_accordion_item title=”Which Hikarazine\u2122 should I use?” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}” open=”off”]<\/p>\n
The choice between Hikarazine\u2122 Z108 and Hikarazine\u2122 Z103 depends on your specific assay.<\/p>\n
[\/et_pb_accordion_item][et_pb_accordion_item title=”Which buffer should I use with Hikarazine\u2122?” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}” open=”off”]<\/p>\n
The choice of buffer is crucial for optimal bioluminescence. PBS is recommended, but other buffers may be used depending on the assay. For instance, adding 0.01% Tween can help with complex matrices, though it may slightly increase background noise. Additionally, varying cation compositions can influence both brightness and bioluminescence half-life. If you need further guidance after testing in your specific assay, please contact our experts.<\/p>\n
[\/et_pb_accordion_item][et_pb_accordion_item title=”What factors influence the light emission process and brightness?” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}” open=”off”]<\/p>\n
The light emission and brightness of Hikarazine\u2122 are influenced by several factors:<\/p>\n
These factors must be optimized to ensure the best performance of the bioluminescent system using Hikarazine\u2122.<\/p>\n
[\/et_pb_accordion_item][et_pb_accordion_item title=”What are the optimal storage conditions for maximum stability of the pro-substrate?” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}” open=”off”]<\/p>\n
To ensure stability for up to two years, store the vials at room temperature, away from heat, light, and humidity.<\/p>\n
[\/et_pb_accordion_item][et_pb_accordion_item title=”Why is the pro-substrate powder darkening?” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}” open=”off”]<\/p>\n
A slight darkening of the surface of the solid may occur over time. However, this does not affect the bioluminescence signal, provided the pro-substrate is used under standard conditions recommended in the IFU.<\/p>\n
[\/et_pb_accordion_item][et_pb_accordion_item title=”What are the optimal storage conditions for the stock solution?” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}” open=”off”]<\/p>\n
The stock solution is acidic, which enhances its stability. When stored at 4\u00b0C, it remains stable for 6 months. For extended stability, it can be stored at -20\u00b0C, with ongoing tests to determine the exact duration.<\/p>\n
[\/et_pb_accordion_item][et_pb_accordion_item title=”What is the peak emission of Hikarazine\u2122?” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}” open=”off”]<\/p>\n
The peak bioluminescence emission of activated luciferins Hikarazine\u2122 Z103 and Z108 has been measured at 450 \u00b1 3 nm.<\/span><\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”What are the recommendations for small animal imaging?” _builder_version=”4.27.6″ _module_preset=”default” global_colors_info=”{}” open=”off”]<\/p>\n Hikarazine\u2122 substrates contain fluor atoms, enhancing their solubility and bioavailability for small animal imaging. [\/et_pb_accordion_item][et_pb_accordion_item title=”Is Hikarazine\u2122 dependent on ATP?” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}” open=”off”]<\/p>\n No, unlike firefly-based bioluminescent systems, marine-based bioluminescent systems, including Hikarazine\u2122, do not require ATP.<\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”Are Hikarazine\u2122 substrates patented?” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}” open=”off”]<\/p>\n Yes, Hikarazine\u2122substrates are patented under EP3615516, US11938199, and JP7366752.<\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”Are Hikarazine\u2122 substrates compatible with all bioluminescence detection instruments ?” _builder_version=”4.27.8″ _module_preset=”default” global_colors_info=”{}” open=”off”]<\/p>\n Yes, Hikarazine\u2122 substrates are compatible with most bioluminescence detection systems, including luminometers, plate readers and in vivo<\/em> imaging systems, including Berthold Technologies instruments, as well as other major detection platforms.<\/span><\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”Is cell lysis required for bioluminescence measurements with Hikarazine\u2122 substrates ?” _builder_version=”4.27.8″ _module_preset=”default” global_colors_info=”{}” open=”off”]<\/p>\n No, thanks to the solubility of the substrates, Hikarazine\u2122 can be added directly to the cell culture medium, allowing bioluminescence measurements to be performed in live cells. <\/span><\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”To perform in vivo assays, how long after injection should I start the bioluminescence acquisition ?” _builder_version=”4.27.8″ _module_preset=”default” global_colors_info=”{}” open=”off”]<\/p>\n For the activated form of Hikarazine\u2122 substrates, we recommend starting bioluminescence acquisitions 5 minutes after injection. <\/span><\/p>\n [\/et_pb_accordion_item][\/et_pb_accordion][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.16″ _module_preset=”default” background_color=”#D2C6D2″ background_enable_image=”off” background_size=”initial” global_colors_info=”{}”][et_pb_row column_structure=”1_2,1_2″ make_equal=”on” _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_column type=”1_2″ module_class=”centrer-verticalement” _builder_version=”4.16″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.16″ _module_preset=”default” text_font=”Rufina||||||||” text_text_color=”#4D1749″ text_font_size=”46px” text_line_height=”40px” custom_margin=”||0px||false|false” text_font_size_tablet=”37px” text_font_size_phone=”35px” text_font_size_last_edited=”on|phone” global_colors_info=”{}”]<\/p>\n We look [\/et_pb_text][\/et_pb_column][et_pb_column type=”1_2″ module_class=”centrer-verticalement” _builder_version=”4.16″ 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global_colors_info=”{}”][\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":" Bioluminescence FAQFind answers to frequently asked questions about bioluminescence, luciferases and bioluminescent substrates, including their use in assays and imaging for life science research.Hikarazine\u2122 has been specifically designed for use with luciferase enzymes derived from Oplophorus gracilirostris.The choice between Hikarazine\u2122 Z108 and Hikarazine\u2122 Z103 depends on your specific assay. For applications where maximizing light output […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","content-type":"","_surecart_dashboard_logo_width":"180px","_surecart_dashboard_show_logo":true,"_surecart_dashboard_navigation_orders":true,"_surecart_dashboard_navigation_invoices":true,"_surecart_dashboard_navigation_subscriptions":true,"_surecart_dashboard_navigation_downloads":true,"_surecart_dashboard_navigation_billing":true,"_surecart_dashboard_navigation_account":true,"footnotes":""},"dipi_cpt_category":[],"folder":[53],"class_list":["post-27397062","page","type-page","status-publish","hentry"],"yoast_head":"\n
For optimal results, we recommend injecting 100 to 150 \u00b5L of Z103 or Z108 at a concentration of 1 mM (target dose of 3 mg\/kg for mice weighing approximately 20 g).<\/p>\n
forward to
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a protein
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