{"id":2002,"date":"2022-03-09T13:57:10","date_gmt":"2022-03-09T13:57:10","guid":{"rendered":"http:\/\/synthelis.com\/etude-de-faisabilite\/"},"modified":"2025-02-25T17:51:16","modified_gmt":"2025-02-25T17:51:16","slug":"feasibility-study","status":"publish","type":"page","link":"https:\/\/synthelis.com\/en\/feasibility-study\/","title":{"rendered":"Feasibility study"},"content":{"rendered":"<p>[et_pb_section fb_built=&#8221;1&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; custom_padding=&#8221;0px||0px||true|false&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_row column_structure=&#8221;3_5,2_5&#8243; use_custom_gutter=&#8221;on&#8221; gutter_width=&#8221;1&#8243; make_equal=&#8221;on&#8221; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; width=&#8221;100%&#8221; max_width=&#8221;100%&#8221; min_height=&#8221;50vh&#8221; custom_padding=&#8221;0px||0px||false|false&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;3_5&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; background_image=&#8221;http:\/\/synthelis.com\/wp-content\/uploads\/2022\/03\/Synthelis-Services-Etude-Faisabilite.jpg&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; text_font=&#8221;PT Serif||||||||&#8221; text_text_color=&#8221;#FFFFFF&#8221; text_font_size=&#8221;16px&#8221; background_layout=&#8221;dark&#8221; min_height=&#8221;33vh&#8221; custom_margin=&#8221;0px||||false|false&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_text][\/et_pb_column][et_pb_column type=&#8221;2_5&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; use_background_color_gradient=&#8221;on&#8221; background_color_gradient_direction=&#8221;45deg&#8221; background_color_gradient_stops=&#8221;#29102e 6%|#4d1749 65%&#8221; background_color_gradient_start=&#8221;#29102e&#8221; background_color_gradient_start_position=&#8221;6%&#8221; background_color_gradient_end=&#8221;#4d1749&#8243; background_color_gradient_end_position=&#8221;65%&#8221; custom_padding=&#8221;78px|65px|78px|65px|true|true&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.16.0&#8243; _module_preset=&#8221;default&#8221; text_font=&#8221;Rufina||||||||&#8221; text_text_color=&#8221;#FFFFFF&#8221; text_font_size=&#8221;4em&#8221; text_line_height=&#8221;1em&#8221; header_font=&#8221;Rufina||||||||&#8221; header_text_color=&#8221;#FFFFFF&#8221; header_font_size=&#8221;55px&#8221; header_font_size_tablet=&#8221;55px&#8221; header_font_size_phone=&#8221;40px&#8221; header_font_size_last_edited=&#8221;on|phone&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<h1>Feasibility study.<\/h1>\n<p>[\/et_pb_text][et_pb_divider show_divider=&#8221;off&#8221; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; width=&#8221;100%&#8221; height=&#8221;50px&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_divider][et_pb_text _builder_version=&#8221;4.16.0&#8243; _module_preset=&#8221;default&#8221; text_font=&#8221;PT Serif||||||||&#8221; text_text_color=&#8221;#FFFFFF&#8221; text_font_size=&#8221;16px&#8221; background_layout=&#8221;dark&#8221; custom_margin=&#8221;0px||||false|false&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p>We are protein biodesigners<\/p>\n<p>[\/et_pb_text][et_pb_divider show_divider=&#8221;off&#8221; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; width=&#8221;100%&#8221; height=&#8221;50px&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_divider][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=&#8221;1&#8243; module_id=&#8221;introduction&#8221; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; background_color=&#8221;#EDE8EC&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_row _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; custom_margin=&#8221;25px||25px||true|false&#8221; custom_padding=&#8221;||||false|false&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;4_4&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.16.0&#8243; _module_preset=&#8221;default&#8221; text_font=&#8221;PT Serif||||||||&#8221; text_text_color=&#8221;#4D1749&#8243; text_font_size=&#8221;1.9em&#8221; text_line_height=&#8221;1.3em&#8221; text_font_size_tablet=&#8221;1.7em&#8221; text_font_size_phone=&#8221;1.6em&#8221; text_font_size_last_edited=&#8221;on|phone&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p class=\"p1\">Each new protein is an individual project and an exciting new challenge for our team. To succeed, this protein production project must be effectively designed from the onset.<br \/>We first follow a rigorous process, a feasibility study, to ensure that our customers are fully satisfied with their protein of interest.<\/p>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=&#8221;1&#8243; module_id=&#8221;introduction&#8221; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; background_color=&#8221;#FFFFFF&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_row _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;4_4&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.16.0&#8243; _module_preset=&#8221;default&#8221; header_font=&#8221;PT Serif|700|||||||&#8221; header_text_color=&#8221;#4D1749&#8243; header_2_font=&#8221;PT Serif|700|||||||&#8221; header_2_font_size=&#8221;30px&#8221; border_color_bottom=&#8221;#EEEFF3&#8243; global_colors_info=&#8221;{}&#8221;]<\/p>\n<h2>Feasibility study: key stages<\/h2>\n<p>[\/et_pb_text][et_pb_divider color=&#8221;#D2C6D2&#8243; divider_position=&#8221;center&#8221; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; custom_margin=&#8221;0px||0px||true|false&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_divider][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; custom_margin=&#8221;||25px||false|false&#8221; custom_padding=&#8221;||||false|false&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;4_4&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][dnxte_timeline timeline_bar_color=&#8221;#EDE8EC&#8221; _builder_version=&#8221;4.16.0&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][dnxte_timeline_child timeline_title=&#8221;1\/ Discussion of your project and expectations&#8221; timeline_use_image=&#8221;off&#8221; timeline_icon=&#8221;&#xf500;||fa||900&#8243; timeline_use_button=&#8221;off&#8221; timeline_icon_color=&#8221;#FFFFFF&#8221; timeline_bg_color=&#8221;#EDE8EC&#8221; icon_bg_color=&#8221;#4D1749&#8243; _builder_version=&#8221;4.16.0&#8243; _module_preset=&#8221;default&#8221; header_text_color=&#8221;#4D1749&#8243; content_text_color=&#8221;#72486E&#8221; identifier_font=&#8221;|700|||||||&#8221; identifier_text_color=&#8221;#29102E&#8221; background_color=&#8221;RGBA(255,255,255,0)&#8221; background_enable_color=&#8221;on&#8221; box_shadow_style_timeline=&#8221;preset3&#8243; box_shadow_style_icon=&#8221;preset3&#8243; global_colors_info=&#8221;{}&#8221;][\/dnxte_timeline_child][dnxte_timeline_child timeline_title=&#8221;2\/ Preliminary study and quotation&#8221; timeline_use_image=&#8221;off&#8221; timeline_icon=&#8221;&#xe058;||divi||400&#8243; timeline_use_button=&#8221;off&#8221; timeline_icon_color=&#8221;#FFFFFF&#8221; timeline_bg_color=&#8221;#EDE8EC&#8221; icon_bg_color=&#8221;#4D1749&#8243; _builder_version=&#8221;4.16.0&#8243; _module_preset=&#8221;default&#8221; header_text_color=&#8221;#4D1749&#8243; content_text_color=&#8221;#72486E&#8221; identifier_font=&#8221;|700|||||||&#8221; identifier_text_color=&#8221;#29102E&#8221; background_color=&#8221;RGBA(255,255,255,0)&#8221; background_enable_color=&#8221;on&#8221; box_shadow_style_timeline=&#8221;preset3&#8243; box_shadow_style_icon=&#8221;preset3&#8243; global_colors_info=&#8221;{}&#8221;][\/dnxte_timeline_child][dnxte_timeline_child timeline_title=&#8221;3\/ Option &#8211; In silico engineering&#8221; timeline_use_image=&#8221;off&#8221; timeline_icon=&#8221;&#xf0c3;||fa||900&#8243; timeline_use_button=&#8221;off&#8221; timeline_icon_color=&#8221;#FFFFFF&#8221; timeline_bg_color=&#8221;#EDE8EC&#8221; icon_bg_color=&#8221;#4D1749&#8243; _builder_version=&#8221;4.16.0&#8243; _module_preset=&#8221;default&#8221; header_text_color=&#8221;#4D1749&#8243; content_text_color=&#8221;#72486E&#8221; identifier_font=&#8221;|700|||||||&#8221; identifier_text_color=&#8221;#29102E&#8221; background_color=&#8221;RGBA(255,255,255,0)&#8221; background_enable_color=&#8221;on&#8221; box_shadow_style_timeline=&#8221;preset3&#8243; box_shadow_style_icon=&#8221;preset3&#8243; global_colors_info=&#8221;{}&#8221;][\/dnxte_timeline_child][dnxte_timeline_child timeline_title=&#8221;4\/ Development of the expression vector(s)&#8221; timeline_use_image=&#8221;off&#8221; timeline_icon=&#8221;&#xf492;||fa||900&#8243; timeline_use_button=&#8221;off&#8221; timeline_icon_color=&#8221;#FFFFFF&#8221; timeline_bg_color=&#8221;#EDE8EC&#8221; icon_bg_color=&#8221;#4D1749&#8243; _builder_version=&#8221;4.16.0&#8243; _module_preset=&#8221;default&#8221; header_text_color=&#8221;#4D1749&#8243; content_text_color=&#8221;#72486E&#8221; identifier_font=&#8221;|700|||||||&#8221; identifier_text_color=&#8221;#29102E&#8221; background_color=&#8221;RGBA(255,255,255,0)&#8221; background_enable_color=&#8221;on&#8221; box_shadow_style_timeline=&#8221;preset3&#8243; box_shadow_style_icon=&#8221;preset3&#8243; global_colors_info=&#8221;{}&#8221;][\/dnxte_timeline_child][dnxte_timeline_child timeline_title=&#8221;5\/ Screening of expression and purification conditions&#8221; timeline_use_image=&#8221;off&#8221; timeline_icon=&#8221;&#xe05a;||fa||900&#8243; timeline_use_button=&#8221;off&#8221; timeline_icon_color=&#8221;#FFFFFF&#8221; timeline_bg_color=&#8221;#EDE8EC&#8221; icon_bg_color=&#8221;#4D1749&#8243; _builder_version=&#8221;4.16.0&#8243; _module_preset=&#8221;default&#8221; header_text_color=&#8221;#4D1749&#8243; content_text_color=&#8221;#72486E&#8221; identifier_font=&#8221;|700|||||||&#8221; identifier_text_color=&#8221;#29102E&#8221; background_color=&#8221;RGBA(255,255,255,0)&#8221; background_enable_color=&#8221;on&#8221; box_shadow_style_timeline=&#8221;preset3&#8243; box_shadow_style_icon=&#8221;preset3&#8243; global_colors_info=&#8221;{}&#8221;][\/dnxte_timeline_child][dnxte_timeline_child timeline_title=&#8221;6\/ Feasibility study and dispatch of sample(s)&#8221; timeline_use_image=&#8221;off&#8221; timeline_icon=&#8221;&#xe059;||divi||400&#8243; timeline_use_button=&#8221;off&#8221; timeline_icon_color=&#8221;#FFFFFF&#8221; timeline_bg_color=&#8221;#EDE8EC&#8221; icon_bg_color=&#8221;#4D1749&#8243; _builder_version=&#8221;4.16.0&#8243; _module_preset=&#8221;default&#8221; header_text_color=&#8221;#4D1749&#8243; content_text_color=&#8221;#72486E&#8221; identifier_font=&#8221;|700|||||||&#8221; identifier_text_color=&#8221;#29102E&#8221; background_color=&#8221;RGBA(255,255,255,0)&#8221; background_enable_color=&#8221;on&#8221; box_shadow_style_timeline=&#8221;preset3&#8243; box_shadow_style_icon=&#8221;preset3&#8243; global_colors_info=&#8221;{}&#8221;][\/dnxte_timeline_child][\/dnxte_timeline][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=&#8221;1&#8243; module_id=&#8221;introduction&#8221; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; background_color=&#8221;#EDE8EC&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_row _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;4_4&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.16.0&#8243; _module_preset=&#8221;default&#8221; header_font=&#8221;PT Serif|700|||||||&#8221; header_text_color=&#8221;#4D1749&#8243; header_3_font=&#8221;PT Serif|700|||||||&#8221; header_3_font_size=&#8221;30px&#8221; border_color_bottom=&#8221;#EEEFF3&#8243; global_colors_info=&#8221;{}&#8221;]<\/p>\n<h3>Discussion of your project and your expectations<\/h3>\n<p>[\/et_pb_text][et_pb_divider color=&#8221;#D2C6D2&#8243; divider_position=&#8221;center&#8221; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; custom_margin=&#8221;0px||0px||true|false&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_divider][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=&#8221;1_2,1_2&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; custom_margin=&#8221;||||true|false&#8221; custom_padding=&#8221;||||false|false&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;1_2&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.16.0&#8243; _module_preset=&#8221;default&#8221; text_font=&#8221;PT Serif||||||||&#8221; text_text_color=&#8221;#4D1749&#8243; text_font_size=&#8221;1.4em&#8221; text_line_height=&#8221;1.4em&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p class=\"p1\"><span style=\"color: #000000;\">As soon as you have informed us of your request, we will set up an initial telephone or video meeting to discuss your project, in order to fully understand your needs and objectives. A confidentiality agreement can be put in place, if necessary at this stage.<\/span><\/p>\n<p class=\"p1\">[\/et_pb_text][\/et_pb_column][et_pb_column type=&#8221;1_2&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][dnxte_button button_text=&#8221;Contact us&#8221; button_link=&#8221;@ET-DC@eyJkeW5hbWljIjp0cnVlLCJjb250ZW50IjoicG9zdF9saW5rX3VybF9wYWdlIiwic2V0dGluZ3MiOnsicG9zdF9pZCI6IjI2NCJ9fQ==@&#8221; dnxt_button_alignment=&#8221;center&#8221; btn_show_hide=&#8221;off&#8221; dnxt_hover_2d=&#8221;dnxt-hover-float&#8221; dnxt_hover_bg_color=&#8221;#4D1749&#8243; _builder_version=&#8221;4.27.4&#8243; _dynamic_attributes=&#8221;button_link&#8221; _module_preset=&#8221;default&#8221; btn_fonts_font=&#8221;PT Serif||||||||&#8221; btn_fonts_text_color=&#8221;#FFFFFF&#8221; background_color=&#8221;#29102E&#8221; use_background_color_gradient=&#8221;on&#8221; background_color_gradient_type=&#8221;linear&#8221; background_color_gradient_direction=&#8221;-90deg&#8221; background_color_gradient_stops=&#8221;#29102e 0%|#4d1749 100%&#8221; background_color_gradient_start=&#8221;#29102e&#8221; background_color_gradient_end=&#8221;#4d1749&#8243; custom_margin=&#8221;||40px||false|false&#8221; custom_padding=&#8221;20px|60px|20px|60px|true|true&#8221; hover_enabled=&#8221;0&#8243; border_width_all_btn_border=&#8221;0px&#8221; box_shadow_style=&#8221;preset3&#8243; global_colors_info=&#8221;{}&#8221; border_color_all_btn_border=&#8221;RGBA(255,255,255,0)&#8221; sticky_enabled=&#8221;0&#8243;][\/dnxte_button][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;4_4&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.16.0&#8243; _module_preset=&#8221;default&#8221; header_font=&#8221;PT Serif|700|||||||&#8221; header_text_color=&#8221;#4D1749&#8243; header_3_font=&#8221;PT Serif|700|||||||&#8221; header_3_font_size=&#8221;30px&#8221; border_color_bottom=&#8221;#EEEFF3&#8243; global_colors_info=&#8221;{}&#8221;]<\/p>\n<h3>Preliminary study and quotation<\/h3>\n<p>[\/et_pb_text][et_pb_divider color=&#8221;#D2C6D2&#8243; divider_position=&#8221;center&#8221; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; custom_margin=&#8221;0px||0px||true|false&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_divider][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=&#8221;1_2,1_2&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; custom_margin=&#8221;||||true|false&#8221; custom_padding=&#8221;||||false|false&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;1_2&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.16.0&#8243; _module_preset=&#8221;default&#8221; text_font=&#8221;PT Serif||||||||&#8221; text_text_color=&#8221;#4D1749&#8243; text_font_size=&#8221;1.4em&#8221; text_line_height=&#8221;1.4em&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p class=\"p1\"><span style=\"color: #000000;\"><span style=\"color: #4d1749;\"><strong>Literature review and sequence analysis<\/strong><\/span><br \/>Based on the information given by the client, we will search the literature and\/or analyze the articles provided to identify the necessary conditions and the system that will work best to express your protein of interest. We will then gather all the conditions that have a positive impact on the expression of the protein as well as assess any potential difficulties that we may encounter.<br \/>Using protein databases and our sequence analysis tools, we will establish, based on this preliminary study, a work plan, and an estimate of costs to quickly and efficiently evaluate our ability to produce your protein of interest.<\/span><\/p>\n<p class=\"p1\">[\/et_pb_text][\/et_pb_column][et_pb_column type=&#8221;1_2&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.16.0&#8243; _module_preset=&#8221;default&#8221; text_font=&#8221;PT Serif||||||||&#8221; text_text_color=&#8221;#4D1749&#8243; text_font_size=&#8221;1.4em&#8221; text_line_height=&#8221;1.4em&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p class=\"p1\"><span style=\"color: #000000;\"><span style=\"color: #4d1749;\"><strong>Optional: in-silico protein engineering<\/strong><\/span><br \/>\nSynthelis has developed extensive partnerships with artificial intelligence companies which have developed proprietary solutions to predict in-silico the characteristics of the proteins. By leveraging these relationships, we can generate very quickly, and effectively, a library of mutants that will have the specific or optimized property you are looking for in your product or reagent: increased stability, improved activity, stronger affinity, better specificity etc.<\/span><\/p>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;4_4&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.16.0&#8243; _module_preset=&#8221;default&#8221; header_font=&#8221;PT Serif|700|||||||&#8221; header_text_color=&#8221;#4D1749&#8243; header_3_font=&#8221;PT Serif|700|||||||&#8221; header_3_font_size=&#8221;30px&#8221; border_color_bottom=&#8221;#EEEFF3&#8243; global_colors_info=&#8221;{}&#8221;]<\/p>\n<h3>Design and construction of the expression vector<\/h3>\n<p>[\/et_pb_text][et_pb_divider color=&#8221;#D2C6D2&#8243; divider_position=&#8221;center&#8221; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; custom_margin=&#8221;0px||0px||true|false&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_divider][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=&#8221;1_2,1_2&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; custom_margin=&#8221;||||true|false&#8221; custom_padding=&#8221;||||false|false&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;1_2&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.16.0&#8243; _module_preset=&#8221;default&#8221; text_font=&#8221;PT Serif||||||||&#8221; text_text_color=&#8221;#4D1749&#8243; text_font_size=&#8221;1.4em&#8221; text_line_height=&#8221;1.4em&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p class=\"p1\"><span style=\"color: #000000;\">The design of the DNA construct is also a critical stage in the process; one where the experience of a specialist plays a key role and increases the chances of success for a protein production project.<br \/>The right choice of expression tag (6xHis, Avi, Strep etc.), its positioning in relation to the gene, the size of the sequence between the tag and the coding region, the selection of the promoter etc, are all factors influencing the level of expression, the purification and the correct folding of the protein.<\/span><\/p>\n<p class=\"p1\">[\/et_pb_text][\/et_pb_column][et_pb_column type=&#8221;1_2&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.16.0&#8243; _module_preset=&#8221;default&#8221; text_font=&#8221;PT Serif||||||||&#8221; text_text_color=&#8221;#4D1749&#8243; text_font_size=&#8221;1.4em&#8221; text_line_height=&#8221;1.4em&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p class=\"p1\"><span style=\"color: #000000;\">With more than 11 years of experience in protein production and a network of partners specialized in molecular biology, we can identify the DNA construct(s) that will enable the optimal expression and purification of your protein of interest.<\/span><\/p>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;4_4&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.16.0&#8243; _module_preset=&#8221;default&#8221; header_font=&#8221;PT Serif|700|||||||&#8221; header_text_color=&#8221;#4D1749&#8243; header_3_font=&#8221;PT Serif|700|||||||&#8221; header_3_font_size=&#8221;30px&#8221; border_color_bottom=&#8221;#EEEFF3&#8243; global_colors_info=&#8221;{}&#8221;]<\/p>\n<h3>Screening of expression conditions<\/h3>\n<p>[\/et_pb_text][et_pb_divider color=&#8221;#D2C6D2&#8243; divider_position=&#8221;center&#8221; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; custom_margin=&#8221;0px||0px||true|false&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_divider][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=&#8221;1_2,1_2&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; custom_margin=&#8221;||||true|false&#8221; custom_padding=&#8221;||||false|false&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;1_2&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.16.0&#8243; _module_preset=&#8221;default&#8221; text_font=&#8221;PT Serif||||||||&#8221; text_text_color=&#8221;#4D1749&#8243; text_font_size=&#8221;1.4em&#8221; text_line_height=&#8221;1.4em&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p class=\"p1\"><span style=\"color: #000000;\">Once we have a ready-to-use DNA construct, we will test the different key parameters for each expression system used:<\/span><br \/><span style=\"color: #000000;\">\u2013 Salt concentration, cell extract type, T\u00b0C etc. for the cell-free protein synthesis system (CFPS).<\/span><br \/><span style=\"color: #000000;\">\u2013 T\u00b0C, medium, clones etc, for E. coli based systems<\/span><br \/><span style=\"color: #000000;\">The combination of parameters giving the best expression level and a whole protein, soluble if necessary, will be selected at this stage by Western Blot analysis.<\/span><br \/><span style=\"color: #000000;\">\u2013 Estimation of the expression yield.<\/span><\/p>\n<p>[\/et_pb_text][\/et_pb_column][et_pb_column type=&#8221;1_2&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.16.0&#8243; _module_preset=&#8221;default&#8221; text_font=&#8221;PT Serif||||||||&#8221; text_text_color=&#8221;#4D1749&#8243; text_font_size=&#8221;1.4em&#8221; text_line_height=&#8221;1.4em&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p class=\"p1\"><span style=\"color: #000000;\">When the best expression conditions are defined, a small-scale production is carried out to allow the development of the purification protocol and to estimate the production yield of the protein. The resulting purified product is then either shipped to the customer for testing or used for quality control testing at Synthelis.<\/span><\/p>\n<p>[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=&#8221;1&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; background_color=&#8221;#FFFFFF&#8221; background_enable_image=&#8221;off&#8221; background_size=&#8221;initial&#8221; custom_margin=&#8221;||||false|false&#8221; custom_padding=&#8221;||80px||false|false&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_row _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;4_4&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.16.0&#8243; _module_preset=&#8221;default&#8221; text_font=&#8221;PT Serif|700|||||||&#8221; text_font_size=&#8221;30px&#8221; header_font=&#8221;PT Serif|700|||||||&#8221; header_text_color=&#8221;#4D1749&#8243; header_3_font=&#8221;PT Serif|700|||||||&#8221; header_3_font_size=&#8221;30px&#8221; border_color_bottom=&#8221;#EEEFF3&#8243; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p>Find our services on:<\/p>\n<p>[\/et_pb_text][et_pb_divider color=&#8221;#D2C6D2&#8243; divider_position=&#8221;center&#8221; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; custom_margin=&#8221;0px||0px||true|false&#8221; global_colors_info=&#8221;{}&#8221;][\/et_pb_divider][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=&#8221;1_3,1_3,1_3&#8243; make_equal=&#8221;on&#8221; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;1_3&#8243; module_class=&#8221;centrer-verticalement&#8221; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_image src=&#8221;http:\/\/synthelis.com\/wp-content\/uploads\/2022\/03\/Scientist.jpg&#8221; alt=&#8221;Scientist.com logo&#8221; title_text=&#8221;Scientist.com logo&#8221; url=&#8221;https:\/\/scientist.com\/&#8221; url_new_window=&#8221;on&#8221; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; box_shadow_style=&#8221;preset3&#8243; global_colors_info=&#8221;{}&#8221;][\/et_pb_image][\/et_pb_column][et_pb_column type=&#8221;1_3&#8243; module_class=&#8221;centrer-verticalement&#8221; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_image src=&#8221;http:\/\/synthelis.com\/wp-content\/uploads\/2022\/03\/ScienceExchange.jpg&#8221; alt=&#8221;Science Exchange Logo&#8221; title_text=&#8221;Science Exchange Logo&#8221; url=&#8221;https:\/\/ww2.scienceexchange.com\/s\/&#8221; url_new_window=&#8221;on&#8221; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; box_shadow_style=&#8221;preset3&#8243; global_colors_info=&#8221;{}&#8221;][\/et_pb_image][\/et_pb_column][et_pb_column type=&#8221;1_3&#8243; module_class=&#8221;centrer-verticalement&#8221; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_image src=&#8221;http:\/\/synthelis.com\/wp-content\/uploads\/2022\/03\/Labtoo.jpg&#8221; alt=&#8221;Labtoo Logo&#8221; title_text=&#8221;Labtoo Logo&#8221; url=&#8221;https:\/\/www.labtoo.com\/fr\/&#8221; url_new_window=&#8221;on&#8221; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; box_shadow_style=&#8221;preset3&#8243; global_colors_info=&#8221;{}&#8221;][\/et_pb_image][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=&#8221;1&#8243; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; background_color=&#8221;#D2C6D2&#8243; background_enable_image=&#8221;off&#8221; background_size=&#8221;initial&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_row column_structure=&#8221;1_2,1_2&#8243; make_equal=&#8221;on&#8221; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_column type=&#8221;1_2&#8243; module_class=&#8221;centrer-verticalement&#8221; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][et_pb_text _builder_version=&#8221;4.16.0&#8243; _module_preset=&#8221;default&#8221; text_font=&#8221;Rufina||||||||&#8221; text_text_color=&#8221;#4D1749&#8243; text_font_size=&#8221;46px&#8221; text_line_height=&#8221;40px&#8221; custom_margin=&#8221;||0px||false|false&#8221; text_font_size_tablet=&#8221;37px&#8221; text_font_size_phone=&#8221;35px&#8221; text_font_size_last_edited=&#8221;on|phone&#8221; global_colors_info=&#8221;{}&#8221;]<\/p>\n<p>We look<br \/>forward to<br \/>your questions<\/p>\n<p>[\/et_pb_text][\/et_pb_column][et_pb_column type=&#8221;1_2&#8243; module_class=&#8221;centrer-verticalement&#8221; _builder_version=&#8221;4.16&#8243; _module_preset=&#8221;default&#8221; global_colors_info=&#8221;{}&#8221;][dnxte_button button_text=&#8221;Contact us&#8221; button_link=&#8221;@ET-DC@eyJkeW5hbWljIjp0cnVlLCJjb250ZW50IjoicG9zdF9saW5rX3VybF9wYWdlIiwic2V0dGluZ3MiOnsicG9zdF9pZCI6IjI2NCJ9fQ==@&#8221; btn_show_hide=&#8221;off&#8221; dnxt_hover_2d=&#8221;dnxt-hover-float&#8221; dnxt_hover_bg_color=&#8221;#4D1749&#8243; _builder_version=&#8221;4.27.4&#8243; _dynamic_attributes=&#8221;button_link&#8221; _module_preset=&#8221;default&#8221; btn_fonts_font=&#8221;PT Serif||||||||&#8221; btn_fonts_text_color=&#8221;#FFFFFF&#8221; background_color=&#8221;#29102E&#8221; use_background_color_gradient=&#8221;on&#8221; background_color_gradient_type=&#8221;linear&#8221; background_color_gradient_direction=&#8221;-90deg&#8221; background_color_gradient_stops=&#8221;#29102e 0%|#4d1749 100%&#8221; background_color_gradient_start=&#8221;#29102e&#8221; background_color_gradient_end=&#8221;#4d1749&#8243; custom_margin=&#8221;||||false|false&#8221; custom_padding=&#8221;20px|60px|20px|60px|true|true&#8221; hover_enabled=&#8221;0&#8243; border_width_all_btn_border=&#8221;0px&#8221; 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